In vitro transport and uptake of protohypericin and hypericin in the Caco-2 model

The intestinal absorption characteristics of protohypericin, a protonaphthodianthrone present in Hypericum extract, were studied and compared with those of hypericin. The Caco-2 model was used as a model of the intestinal mucosa to assess transepithelial transport and cell uptake. The experimental w...

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Bibliographic Details
Published in:International journal of pharmaceutics 1999-10, Vol.188 (1), p.81-86
Main Authors: Kamuhabwa, A.R, Augustijns, P, de Witte, P.A
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biological Transport - radiation effects
Caco-2
Caco-2 Cells - metabolism
Caco-2 Cells - radiation effects
Cell physiology
Dose-Response Relationship, Drug
Fundamental and applied biological sciences. Psychology
General pharmacology
Humans
Hypericin
Hypericum
Light
Medical sciences
Membrane and intracellular transports
Molecular and cellular biology
Perylene - analogs & derivatives
Perylene - pharmacokinetics
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Plant Extracts - pharmacokinetics
Plants, Medicinal
Protohypericin
Time Factors
Transport
Uptake
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Summary:The intestinal absorption characteristics of protohypericin, a protonaphthodianthrone present in Hypericum extract, were studied and compared with those of hypericin. The Caco-2 model was used as a model of the intestinal mucosa to assess transepithelial transport and cell uptake. The experimental work was performed in specific light conditions that prevented both the photoconversion of protohypericin into hypericin and the photosensitization of the cells. Following application of the individual compounds (80–200 μM) to the apical side of the monolayers, the appearance in the basolateral compartment was found to be very low (
ISSN:0378-5173
1873-3476
DOI:10.1016/S0378-5173(99)00203-3