Chitosan accelerates the production of osteopontin from polymorphonuclear leukocytes

Chitosan is a copolymer of β(1→4) glucosamine and N-acetyl- d-glucosamine, which accelerates the infiltration of polymorphonuclear leukocytes (PMN) in the early phase of wound healing. In the granulation tissue treated with chitosan in canine experimental wound, osteopontin (OPN) was strongly positi...

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Bibliographic Details
Published in:Biomaterials 2001-06, Vol.22 (12), p.1667-1673
Main Authors: Ueno, Hiroshi, Murakami, Masaaki, Okumura, Masahiro, Kadosawa, Tsuyoshi, Uede, Toshimitsu, Fujinaga, Toru
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Chitin - analogs & derivatives
Chitin - pharmacology
Chitosan
Dogs
Gene Expression Regulation - drug effects
Humans
In Vitro Techniques
Kinetics
Medical sciences
Neutrophils - drug effects
Neutrophils - physiology
Osteopontin
Phosphoproteins - blood
Phosphoproteins - genetics
Polymorphonuclear leukocyte
Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - blood
RNA, Messenger - genetics
Sialoglycoproteins - blood
Sialoglycoproteins - genetics
Skin - drug effects
Skin - physiopathology
Skin Physiological Phenomena - drug effects
Technology. Biomaterials. Equipments. Material. Instrumentation
Time Factors
Transcription, Genetic - drug effects
Wound healing
Wound Healing - drug effects
Wound Healing - physiology
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Summary:Chitosan is a copolymer of β(1→4) glucosamine and N-acetyl- d-glucosamine, which accelerates the infiltration of polymorphonuclear leukocytes (PMN) in the early phase of wound healing. In the granulation tissue treated with chitosan in canine experimental wound, osteopontin (OPN) was strongly positive in PMN immunohistochemically. OPN is a glycosylated phosphoprotein and promotes the attachment or spread of a variety of cell types. In addition, OPN may play a role in granulomatous inflammation. Production of OPN in PMN was therefore investigated in vitro using human PMN in this study. PMN stimulated with granulocyte-colony stimulating factor (G-CSF) and chitosan accumulated OPN mRNA, and released OPN into their culture supernatants. These findings suggest that OPN is synthesized by migrating PMN which plays the novel role of regulating the evolution of wound healing with chitosan treatment at the early phase of healing.
ISSN:0142-9612
1878-5905
DOI:10.1016/S0142-9612(00)00328-8