Electronmicroscopical evaluation of short-term nerve regeneration through a thin-walled biodegradable poly(DLLA- ε-CL) nerve guide filled with modified denatured muscle tissue

• Published in: Biomaterials 2001-05, Vol.22 (10), p.1177-1185
• Main Authors: Meek, M.F., Robinson, P.H., Stokroos, I, Blaauw, E.H., Kors, G., den Dunnen, W.F.A.
• Format: Article
• Language: English
• Subjects: Animals; Biocompatible Materials; Electron microscopy; Male; Materials Testing; Microscopy, Electron; Morphometric analysis; Muscle, Skeletal - transplantation; Myelin Sheath - pathology; Myelin Sheath - physiology; Nerve guide; Nerve regeneration; Nerve Regeneration - physiology; Polyesters; Rats; Rats, Wistar; Sciatic Nerve - injuries; Sciatic Nerve - pathology; Sciatic Nerve - physiopathology; Sciatic Nerve - surgery; Time Factors
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/S0142-9612(00)00340-9

The aim of this study was to evaluate short-term peripheral nerve regeneration across a 15-mm gap in the sciatic nerve of the rat, using a thin-walled biodegradable poly( dl-lactide- ε-caprolactone) nerve guide filled with modified denatured muscle tissue (MDMT). The evaluation was performed using transmission electron microscopy and morphometric analysis. Evaluation times ranged from 3 to 12 weeks after reconstruction. Already, 3 weeks after reconstruction, myelinated nerve fibers could be observed in the distal nerve stump. Twelve weeks after reconstruction, the number of (non)myelinated nerve fibers had significantly increased in the distal nerve stump. From this study, we can conclude that a thin-walled biodegradable poly( dl-lactide- ε-caprolactone) nerve guides filled with MDMT can be successfully applied in the reconstruction of severed nerves in the rat model. Furthermore, we showed fast nerve regeneration across the 15-mm nerve gap and found that the use of MDMT functioned as a mechanical support preventing a collapse of this thin-walled nerve guide.