Gene expression of the taurine transporter and taurine biosynthetic enzymes in rat kidney after antidiuresis and salt loading

• Published in: Pflügers Archiv 2001-04, Vol.442 (1), p.87-95
• Main Authors: Bitoun, M, Levillain, O, Tappaz, M
• Format: Article
• Language: English
• Subjects: Aldehyde Reductase - genetics; Animals; Carboxy-Lyases - genetics; Carrier Proteins - genetics; Cysteine Dioxygenase; Dioxygenases; Diuresis; Gene Expression; Heat-Shock Proteins - genetics; Kidney - enzymology; Male; Membrane Glycoproteins - genetics; Membrane Proteins; Membrane Transport Proteins; Oxygenases - genetics; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - analysis; Rodents; Sodium; Sodium Chloride - administration & dosage; Solutions; Sucrose - administration & dosage; Symporters; Taurine - biosynthesis; Tissue Distribution; Water Deprivation
• ISSN: 0031-6768; 1432-2013
• DOI: 10.1007/s004240000506

Taurine is thought to be an osmolyte in the kidney medulla. We have investigated the gene expression of the taurine transporter (TauT) and the enzymes of taurine biosynthesis, cysteine dioxygenase (CDO) and cysteine sulfinate decarboxylase (CSD). We achieved this by measuring their mRNA levels using reverse transcriptase polymerase chain reaction (RT-PCR) in five kidney regions of rats in various hydration states; namely, normal hydration, after 2 days of antidiuresis following chronic diuresis and finally after acute salt loading. The mRNA levels of the well-established tonicity-sensitive genes coding for the aldose reductase (AR), the sodium myo-inositol transporter (SMIT) and the betaine transporter (BGT1) were also determined for the sake of comparison. In normally hydrated rats, TauT-, CDO-, and CSD-mRNA were enriched in the outer stripe of the outer medulla (OS). Following antidiuresis, the mRNA levels of TauT, CDO, CSD, SMIT, BGT1 and AR were all similarly increased in the papilla when compared with levels in rats submitted to a chronic diuresis. After acute salt loading, the mRNA level of TauT, like that of SMIT and BGT1, was overexpressed in OS whereas the mRNA levels of CDO and CSD remained unchanged. Like SMIT, BGT1 and AR genes, TauT, CDO and CSD genes appear to be tonicity-sensitive genes which can be activated in vivo by hypertonicity in the rat kidney. However, tonicity-induced activation of the TauT gene is more sensitive than that of CDO and CSD genes.