Mechanisms underlying the acquisition of resistance to octanoic-acid-induced-death following exposure of Saccharomyces cerevisiae to mild stress imposed by octanoic acid or ethanol

Acquisition of resistance to lethal concentrations of octanoic acid was induced in cells of Saccharomyces cerevisiae grown in the presence of sublethal concentrations of this lipophilic acid or following rapid exposure (1 h) of unadapted yeast cells to mild stress imposed by the same acid. Experimen...

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Bibliographic Details
Published in:Archives of microbiology 2001-04, Vol.175 (4), p.301-307
Main Authors: Cabral, M.G, Viegas, C.A, Sa-Correia, I
Format: Article
Language:English
Subjects:
Action of physical and chemical agents
Adaptation, Physiological
Biological and medical sciences
Caprylates - pharmacology
caprylic acid
cross resistance
death
Enzyme Activation
ethanol
Ethanol - pharmacology
Fundamental and applied biological sciences. Psychology
H-transporting ATP synthase
induced resistance
Microbiology
Mycology
octanoic acid
plasma membrane
protein synthesis
Proton-Translocating ATPases - metabolism
Saccharomyces cerevisiae
Saccharomyces cerevisiae - drug effects
stress response
yeasts
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Summary:Acquisition of resistance to lethal concentrations of octanoic acid was induced in cells of Saccharomyces cerevisiae grown in the presence of sublethal concentrations of this lipophilic acid or following rapid exposure (1 h) of unadapted yeast cells to mild stress imposed by the same acid. Experimental evidence indicated that the referred adaptation involved de novo protein synthesis, presumably due to the rapid induction of a plasma membrane transporter which mediates the active efflux of octanoate out of the cell. Rapid exposure of cells to mild ethanol stress also led to increased resistance to lethal concentrations of octanoic acid. This cross-resistance to octanoic-acid-induced death was below the level of resistance induced by mild octanoic acid stress and did not involve induction of the active expulsion of octanoate out of the cell. However, the rapid exposure of yeast cells to octanoic acid or ethanol led to the activation of plasma membrane H+-ATPase. The physiological role of the two stress responses examined during the present study, namely, the active efflux of octanoate specifically induced by octanoic acid and the stimulation of plasma membrane H+-ATPase activity, is discussed.
ISSN:0302-8933
1432-072X
DOI:10.1007/s002030100269