The synthesis of phosphopeptides

• Published in: Biopolymers 2001, Vol.60 (1), p.3-31
• Main Authors: McMurray, John S., Coleman IV, David R., Wang, Wei, Campbell, Martin L.
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Amino Acids - chemistry; Amino Acids - metabolism; antennapedia homeodomain; cell permeable peptide carriers; dephosphorylation; HIV TAT transcription factor; Organophosphorus Compounds - chemistry; Organophosphorus Compounds - metabolism; Peptides, Cyclic - chemical synthesis; Peptides, Cyclic - chemistry; Peptides, Cyclic - metabolism; phosphatase substrate activity specificity; Phosphates - chemistry; Phosphates - metabolism; phosphoamino acid building blocks; phosphopeptides; Phosphopeptides - chemical synthesis; Phosphopeptides - chemistry; Phosphopeptides - metabolism; Phosphorylation; protein expression; protein regulation; protein-protein interactions; SH2 domain ligand specificity; signal transduction
• ISSN: 0006-3525; 1097-0282
• DOI: 10.1002/1097-0282(2001)60:1<3::AID-BIP1001>3.0.CO;2-L

Phosphorylation and dephosphorylation are key events in protein expression and regulation and in signal transduction. Phosphopeptides are very useful reagents for the study of these processes, and have been used to great advantage in the study of phosphatase substrate specificity, SH2 domain ligand specificity, and protein–protein interactions. Furthermore, the advent of cell‐permeable peptide carriers, such as those from the antennapedia homeodomain and the HIV TAT transcription factor, has allowed the study of intracellular events, thus underscoring the utility of these reagents. In this paper we review methods for the synthesis of phosphopeptides with the emphasis on the preparation of phosphoamino acid building blocks. © 2001 John Wiley & Sons, Inc. Biopolymers (Pept Sci) 60: 3–31, 2001