Crossreactive neutralizing antibodies induced by immunization with caprine arthritis–encephalitis virus surface glycoprotein

Four Saanen goats were immunized with affinity purified gp135 surface glycoprotein (SU) of caprine arthritis–encephalitis virus isolate 79–63 (CAEV-63) and evaluated for homologous and crossreactive serum neutralizing antibodies. CAEV-63 neutralizing antibodies were detected in all goats after seven...

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Bibliographic Details
Published in:Vaccine 2000-01, Vol.18 (13), p.1282-1287
Main Authors: Kemp, R.K., Knowles, D.P., Perry, L.L., McGuire, T.C., Besser, T.E., Cheevers, W.P.
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic - pharmacology
Animals
Antibodies, Monoclonal - metabolism
Antibodies, Viral - biosynthesis
Antibodies, Viral - blood
Antibodies, Viral - metabolism
Antibody Specificity
Arthritis-Encephalitis Virus, Caprine - chemistry
Arthritis-Encephalitis Virus, Caprine - immunology
Arthritis-Encephalitis Virus, Caprine - isolation & purification
Biological and medical sciences
Caprine arthritis encephalitis virus
Caprine arthritis–encephalitis lentivirus surface glycoprotein
Contactin 1
Cross Reactions
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
glycoprotein gp135
Goats
Immunoblotting
Immunoglobulin G - blood
Immunoglobulin G - immunology
Membrane Glycoproteins - immunology
Membrane Glycoproteins - isolation & purification
Microbiology
Nerve Tissue Proteins - immunology
Nerve Tissue Proteins - isolation & purification
Neutralization Tests
Neutralizing antibody responses
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Viral Vaccines - immunology
Virology
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Summary:Four Saanen goats were immunized with affinity purified gp135 surface glycoprotein (SU) of caprine arthritis–encephalitis virus isolate 79–63 (CAEV-63) and evaluated for homologous and crossreactive serum neutralizing antibodies. CAEV-63 neutralizing antibodies were detected in all goats after seven immunizations with SU in Quil A adjuvant. Sera from three goats neutralized an independent CAEV isolate (CAEV-Co). However, serum from one goat did not detectably neutralize heterologous CAEV-Co and inhibited CAEV-Co neutralization by another serum.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(99)00181-4