Fucogalactan isolated from Sarcodon aspratus elicits release of tumor necrosis factor-α and nitric oxide from murine macrophages

Eight species of mushrooms were evaluated for mitogenic activity by the tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method using spleen cells of C3H/HeN female mice. The hot water-soluble (HWS) fraction extracted from Sarcodon aspratus showed the highest acti...

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Bibliographic Details
Published in:Immunopharmacology 2000-02, Vol.46 (2), p.113-121
Main Authors: MIZUNO, M, SHIOMI, Y, MINATO, K.-I, KAWAKAMI, S, ASHIDA, H, TSUCHIDA, H
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic - pharmacology
Agaricales - chemistry
Animals
Biological and medical sciences
Female
Galactans - isolation & purification
Galactans - pharmacology
Immunomodulators
Macrophages - drug effects
Macrophages - secretion
Medical sciences
Mice
Mice, Inbred C3H
Mitogens - pharmacology
Nitric Oxide - biosynthesis
Pharmacology. Drug treatments
Tumor Necrosis Factor-alpha - biosynthesis
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Summary:Eight species of mushrooms were evaluated for mitogenic activity by the tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method using spleen cells of C3H/HeN female mice. The hot water-soluble (HWS) fraction extracted from Sarcodon aspratus showed the highest activity. The mitogen in Sa. aspratus was isolated by Sepharose 6B and DEAE-Sepharose CL-6B column chromatography. Preliminary structural analyses indicated that the mitogen was a fucogalactan. Fucogalactan elicited the release of tumor necrosis factor-alpha (TNF-alpha) and nitric oxide (NO) in macrophages of mice in vitro. TNF-alpha production induced with 50 microg/ml of fucogalactan was significantly higher than that induced by lentinan (500 microg/ml) by approximately 4.3-fold. Also, fucogalactan showed dose dependence at concentrations from 5 to 500 microg/ml in NO production. Thus, fucogalactan does elicit the release of cytokines such as TNF-alpha and NO.
ISSN:0162-3109
DOI:10.1016/S0162-3109(99)00163-0