Infrared Spectroscopic Characterization of the Interaction of Cationic Lipids with Plasmid DNA

Fourier transform infrared spectroscopy was used to characterize the interaction of the cationic lipids 1,2-dioleoyl-3-trimethylammonium-propane and dioctadecyldimethylammonium bromide with plasmid DNA. The effect of incorporating the neutral colipids cholesterol and dioleoylphosphatidylethanolamine...

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Bibliographic Details
Published in:The Journal of biological chemistry 2001-03, Vol.276 (11), p.8037-8043
Main Authors: Choosakoonkriang, Sirirat, Wiethoff, Christopher M., Anchordoquy, Thomas J., Koe, Gary S., Smith, Janet G., Middaugh, C.Russell
Format: Article
Language:English
Subjects:
cholesterol
Cholesterol - pharmacology
Colloids
DNA - chemistry
Glycerophospholipids - pharmacology
Lipids - chemistry
Nucleic Acid Conformation
Phosphatidylethanolamines
Plasmids
Spectroscopy, Fourier Transform Infrared
Vibration
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Summary:Fourier transform infrared spectroscopy was used to characterize the interaction of the cationic lipids 1,2-dioleoyl-3-trimethylammonium-propane and dioctadecyldimethylammonium bromide with plasmid DNA. The effect of incorporating the neutral colipids cholesterol and dioleoylphosphatidylethanolamine on this interaction was also examined. Additionally, dynamic and phase analysis light scattering were used to monitor the size and ζ potential of the resulting complexes under conditions similar to the Fourier transform infrared measurements. Results suggest that upon interaction of cationic lipids with DNA, the DNA remains in the B form. Distinct changes in the frequency of several infrared bands arising from the DNA bases, however, suggest perturbation of their hydration upon interaction with cationic lipids. A direct interaction of the lipid ammonium headgroup with and dehydration of the DNA phosphate is observed when DNA is complexed with these lipids. Changes in the apolar regions of the lipid bilayer are minimal, whereas the interfacial regions of the membrane show changes in hydration or molecular packing. Incorporation of helper lipids into the cationic membranes results in increased conformational disorder of the apolar region and further dehydration of the interfacial region. Changes in the hydration of the DNA bases were also observed as the molar ratio of helper lipid in the membranes was increased.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M010592200