Stir bar sorptive extraction–thermal desorption–capillary gas chromatography–mass spectrometry applied to the analysis of polychlorinated biphenyls in human sperm

Stir bar sorptive extraction (SBSE) on polydimethylsiloxane (PDMS) was applied to the enrichment of polychlorinated biphenyls (PCBs) from human sperm. The seven Ballschmiter PCBs were used as model compounds. The extracted PCBs were then thermally desorbed from the stir bar and analysed on-line by c...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 2001-05, Vol.755 (1), p.137-142
Main Authors: Benijts, Tom, Vercammen, Joeri, Dams, Riet, Tuan, Hai Pham, Lambert, Willy, Sandra, Pat
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Gas Chromatography-Mass Spectrometry - methods
General pharmacology
Humans
Male
Medical sciences
Methanol
Pharmacology. Drug treatments
Polychlorinated biphenyls
Polychlorinated Biphenyls - analysis
Spermatozoa - chemistry
Spermatozoa - metabolism
Water - chemistry
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Summary:Stir bar sorptive extraction (SBSE) on polydimethylsiloxane (PDMS) was applied to the enrichment of polychlorinated biphenyls (PCBs) from human sperm. The seven Ballschmiter PCBs were used as model compounds. The extracted PCBs were then thermally desorbed from the stir bar and analysed on-line by capillary gas chromatography (CGC) with mass spectrometric detection (MS). Method development started with the analysis of PCBs spiked in water. Methanol had to be added to the samples in order to reduce the influence of glass adsorption on recovery and reproducibility. Recoveries in water for all PCBs varied around 50–60% and were limited for low molecular mass (MM) PCBs by polarity changes in the sample due to methanol addition and for high MM PCBs by non-equilibrium conditions. Matrix suppression by the lipophilic medium lowered the recoveries in the sperm samples proportional with PCB polarity. The method was validated and although limits of detection (LOD) for the individual congeners were in the sub-ppt level (
ISSN:0378-4347
1387-2273
DOI:10.1016/S0378-4347(01)00048-2