Biological activities of antibodies elicited by lipooligosaccharide based-conjugate vaccines of nontypeable Haemophilus influenzae in an otitis media model

Vaccination of chinchillas with nontypeable Haemophilus influenzae (NTHi) lipooligosaccharide (LOS) conjugates protected against otitis media. Correlations between the levels of conjugate-induced LOS antibodies (Abs) in sera and middle ear fluids (MEFs) and Ab-mediated biological functions and prote...

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Bibliographic Details
Published in:Vaccine 2000-01, Vol.18 (13), p.1264-1272
Main Authors: Sun, Jianzhong, Chen, Jing, Cheng, Zhengyi, Robbins, John B., Battey, James F., Gu, Xin-Xing
Format: Article
Language:English
Subjects:
Animals
Antibodies, Bacterial - biosynthesis
Antibodies, Bacterial - blood
Antibodies, Blocking - blood
Antibody Specificity
Bacterial diseases
Bacteriology
Biological and medical sciences
Blood Bactericidal Activity - immunology
Chinchilla
Chinchilla laniger
Colony Count, Microbial
Conjugate vaccine
Experimental bacterial diseases and models
Exudates and Transudates - immunology
Exudates and Transudates - microbiology
Fundamental and applied biological sciences. Psychology
Haemophilus influenzae
Haemophilus influenzae - classification
Haemophilus influenzae - immunology
Haemophilus Vaccines - immunology
Haemophilus Vaccines - therapeutic use
Humans
Immune Sera - analysis
Immunoglobulin Isotypes - biosynthesis
Infectious diseases
Lipooligosaccharide
lipooligosaccharides
Lipopolysaccharides - immunology
Lipopolysaccharides - therapeutic use
Medical sciences
Microbiology
Opsonin Proteins - immunology
Otitis media
Otitis Media with Effusion - blood
Otitis Media with Effusion - immunology
Otitis Media with Effusion - prevention & control
Phagocytosis - immunology
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, Conjugate - immunology
Vaccines, Conjugate - therapeutic use
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Summary:Vaccination of chinchillas with nontypeable Haemophilus influenzae (NTHi) lipooligosaccharide (LOS) conjugates protected against otitis media. Correlations between the levels of conjugate-induced LOS antibodies (Abs) in sera and middle ear fluids (MEFs) and Ab-mediated biological functions and protection were examined. Following parenteral vaccination and middle ear challenge, all vaccinated animals, but none of the controls, had high titers of anti-LOS in their sera and MEFs. There was a correlation between the levels of anti-LOS IgG+M, IgG or IgA in the sera and in the MEFs ( P
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(99)00381-3