Recombinant Human Antibodies Specific for the Pfs48/45 Protein of the Malaria Parasite Plasmodium falciparum

We report the first construction of two combinatorial human phage display libraries derived from malaria-immune patients. Specific single-chain Fv fragments (scFv) against Pfs48/45, a gamete surface protein of the sexual stages of Plasmodium falciparum, were selected and analyzed extensively. The se...

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Bibliographic Details
Published in:The Journal of biological chemistry 2001-06, Vol.276 (23), p.19807-19811
Main Authors: Roeffen, Will F.G., Raats, Jos M.H., Teelen, Karina, Hoet, Rene M.A., Eling, Wijnand M., van Venrooij, Walther J., Sauerwein, Robert W.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antibodies - chemistry
Antibodies - genetics
Antibodies - immunology
Bacteriophages - genetics
Epitopes - immunology
Humans
Membrane Glycoproteins - chemistry
Membrane Glycoproteins - immunology
Molecular Sequence Data
Pfs48/45 antigen
Pfs48/45 protein
Plasmodium falciparum
Plasmodium falciparum - immunology
Protozoan Proteins - chemistry
Protozoan Proteins - immunology
Recombinant Proteins - chemistry
Recombinant Proteins - genetics
Recombinant Proteins - immunology
Sequence Homology, Amino Acid
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Summary:We report the first construction of two combinatorial human phage display libraries derived from malaria-immune patients. Specific single-chain Fv fragments (scFv) against Pfs48/45, a gamete surface protein of the sexual stages of Plasmodium falciparum, were selected and analyzed extensively. The selected scFv reacted with the surface of extracellular sexual forms of the parasite and showed Pfs48/45 reactivity on immunoblot. The scFv inhibit binding of human malaria sera to native Pfs48/45 from gametocytes. Moreover, the scFv bind to target epitopes of Pfs48/45 exposed in natural infections. Sequence analysis of eight scFv clones specific for epitope III of Pfs48/45 revealed that these clones could be divided into one VH family-derived germ-line gene (VH1) and two VL family segments (VL2 and VKI).
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M100562200