Determination of trovafloxacin in human serum by time resolved terbium-sensitised luminescence

• Published in: European journal of pharmaceutical sciences 2001-06, Vol.13 (3), p.297-301
• Main Authors: OCANA, Juan A, CALLEJON, Manuel, BARRAGAN, Francisco José
• Format: Article
• Language: English
• Subjects: Analysis; Anti-Infective Agents - blood; Antibacterial agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; Biological and medical sciences; Excipients; Fluoroquinolones; General pharmacology; Indicators and Reagents; Luminescent Measurements; Medical sciences; Naphthyridines - blood; Pharmacology. Drug treatments; Serum; Sodium Dodecyl Sulfate; Spectrometry, Fluorescence; Surface-Active Agents; Terbium - chemistry; Terbium-sensitised luminescence; Trovafloxacin determination
• ISSN: 0928-0987; 1879-0720
• DOI: 10.1016/S0928-0987(01)00116-6

A sensitive time-resolved luminescence method for the determination of trovafloxacin is described. The method is based on the time-resolved luminescence signal from the terbium(III)–trovafloxacin complex, in a micellar solution of sodium dodecyl sulfate (SDS), using a chemical deoxygenation agent (Na 2SO 3). The method allows the determination of 20–450 ng ml −1 of trovafloxacin in 7.5 mM SDS solution containing 0.16 M acetic acid–sodium acetate buffer (pH 6.0) and 7.5 mM Na 2SO 3 with λ exc=270 nm and λ em=546 nm. In these experimental conditions luminescence signal for trovafloxacin increases 20-fold with respect to native fluorescence of the compound in aqueous solution at pH 6.5. Terbium-sensitised luminescence was applied to trovafloxacin determination in human serum, spiked at levels found after drug administration at normal clinical doses. Recovery is 90±1% and day-to-day precision is 3.5%. The proposed method tolerates high concentrations of other co-administrated drugs and excipients.