Direct TLC-MALDI Coupling Using a Hybrid Plate

A new TLC-MALDI direct coupling method which recovers approximately 100% of the analyte is presented. The method makes use of a hybrid TLC-MALDI plate in which a silica layer and a MALDI layer are configured adjacently on a common backing. After TLC separation, the plate is rotated 90° and the separ...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2000-01, Vol.72 (1), p.68-73
Main Authors: Mehl, John T, Hercules, David M
Format: Article
Language:English
Subjects:
Aminoacids, peptides. Hormones. Neuropeptides
Analytical chemistry
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, Thin Layer - methods
Exact sciences and technology
Fluorometry
Fundamental and applied biological sciences. Psychology
Measurement
Methods
Other chromatographic methods
Proteins
Scientific imaging
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
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Summary:A new TLC-MALDI direct coupling method which recovers approximately 100% of the analyte is presented. The method makes use of a hybrid TLC-MALDI plate in which a silica layer and a MALDI layer are configured adjacently on a common backing. After TLC separation, the plate is rotated 90° and the separated analyte spots are eluted from the silica layer to the MALDI layer via capillary action of the MALDI layer. Signal-to-noise ratios are significantly improved over previously reported coupling methods. Low-femtomole detection limits have been demonstrated for small cyclic peptides, which are comparable to detection limits for standard MALDI measurements.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac990003y