Expression of chloramphenicol acetyltransferase in Bacillus subtilis under the control of a phytoplasma promoter

A cloned putative promoter region upstream of the 16S rRNA gene of the western X-disease phytoplasma was inserted behind the promoterless chloramphenicol acetyltransferase gene of plasmid pPL603. The DNA construct was used to transform Bacillus subtilis cells. The transformants were assayed for chlo...

Full description

Saved in:
Bibliographic Details
Published in:FEMS microbiology letters 2001-05, Vol.199 (2), p.177-179
Main Authors: Palmano, S, Kirkpatrick, B.C, Firrao, G
Format: Article
Language:English
Subjects:
16S rRNA gene
Acetyltransferase
Acholeplasmataceae - genetics
Bacillus subtilis
Bacillus subtilis - enzymology
Bacillus subtilis - genetics
Bacteriology
Biological and medical sciences
Chloramphenicol
chloramphenicol acetyltransferase
Chloramphenicol O-acetyltransferase
Chloramphenicol O-Acetyltransferase - genetics
Chloramphenicol O-Acetyltransferase - metabolism
Chloromycetin
Deoxyribonucleic acid
DNA
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Bacterial
Gene Expression Regulation, Enzymologic
genes
Genes, Reporter
Genetic Vectors - genetics
Genetics
Microbiology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Phytoplasma
plasmids
Plasmids - genetics
Promoter
promoter regions
Promoter Regions, Genetic - genetics
Recombination, Genetic
ribosomal RNA
RNA, Ribosomal, 16S - genetics
rRNA 16S
Transformation, Bacterial
Western X phytoplasma
Western X-disease
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A cloned putative promoter region upstream of the 16S rRNA gene of the western X-disease phytoplasma was inserted behind the promoterless chloramphenicol acetyltransferase gene of plasmid pPL603. The DNA construct was used to transform Bacillus subtilis cells. The transformants were assayed for chloramphenicol acetyltransferase activity, showing that the phytoplasma promoter is efficiently expressed in a B. subtilis background.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2001.tb10670.x