Cloning and Expression of the Wnt Antagonists Sfrp-2 and Frzb during Chick Development

The Wnt genes are known to play fundamental roles during patterning and development of a number of embryonic structures. Receptors for Wnts are members of the Frizzled family of proteins containing a cysteine-rich domain (CRD) that binds the Wnt protein. Recently several secreted frizzled-related pr...

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Bibliographic Details
Published in:Developmental biology 2000-02, Vol.218 (2), p.183-198
Main Authors: Ladher, R.K., Church, V.L., Allen, S., Robson, L., Abdelfattah, A., Brown, N.A., Hattersley, G., Rosen, V., Luyten, F.P., Dale, L., Francis-West, P.H.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Chick Embryo
chondrogenesis
Cloning, Molecular
craniofacial
DNA Primers
Embryonic Development
Eye Proteins - genetics
Frzb
Frzb gene
Glycoproteins
heart
limb
Molecular Sequence Data
myogenesis
neural
placodes
Proteins - genetics
Proto-Oncogene Proteins - antagonists & inhibitors
Sequence Homology, Amino Acid
Sfrp-2 (SDF-5)
Sfrp-2 gene
Wnt antagonist
Wnt Proteins
Xenopus
Zebrafish Proteins
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Summary:The Wnt genes are known to play fundamental roles during patterning and development of a number of embryonic structures. Receptors for Wnts are members of the Frizzled family of proteins containing a cysteine-rich domain (CRD) that binds the Wnt protein. Recently several secreted frizzled-related proteins (Sfrps) that also contain a CRD have been identified and some of these can both bind and antagonise Wnt proteins. In this paper we report the expression patterns of the chick homologues of Frzb, a known Wnt antagonist, and Sfrp-2. Both genes are expressed in areas where Wnts are known to play a role in development, including the neural tube, myotome, cartilage, and sites of epithelial–mesenchymal interactions. Initially, Sfrp-2 and Frzb are expressed in overlapping areas in the neural plate and neural tube, whereas later, they have distinct patterns. In particular Sfrp-2 is associated with myogenesis while Frzb is associated with chondrogenesis, suggesting that they play different roles during development. Finally, we have used the early Xenopus embryo as an in vivo assay to show that Sfrp-2, like Frzb, is a Wnt antagonist. These results suggest that Sfrp-2 and Frzb may function in the developing embryo by modulating Wnt signalling.
ISSN:0012-1606
1095-564X
DOI:10.1006/dbio.1999.9586