Analysis of thiocyanate in biological fluids by capillary zone electrophoresis

A new sensitive and simple method has been developed for the determination of thiocyanate in human serum, urine and saliva. The determinations were performed in a fused-silica capillary [64.5 cm (56 cm effective length)×75 μm] using 0.1 M β-alanine–HCl (pH 3.50) as a background electrolyte, separati...

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Bibliographic Details
Published in:Journal of Chromatography A 2001-05, Vol.916 (1), p.273-277
Main Authors: Glatz, Zdeněk, Nováková, Soňa, Štěrbová, Hana
Format: Article
Language:English
Subjects:
Biological and medical sciences
Electrophoresis, Capillary - methods
Food toxicology
Inorganic anions
Medical sciences
Reproducibility of Results
Saliva - chemistry
Sensitivity and Specificity
Spectrophotometry, Ultraviolet
Thiocyanate
Thiocyanates - analysis
Thiocyanates - blood
Thiocyanates - urine
Tobacco, tobacco smoking
Toxicology
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Summary:A new sensitive and simple method has been developed for the determination of thiocyanate in human serum, urine and saliva. The determinations were performed in a fused-silica capillary [64.5 cm (56 cm effective length)×75 μm] using 0.1 M β-alanine–HCl (pH 3.50) as a background electrolyte, separation voltage 18 kV (negative polarity), temperature of capillary 25°C and direct detection at 200 nm. Serum samples were 10-times diluted with deionised water and deproteinised with acetonitrile in the ratio 1:2. Urine and saliva samples need only 20-fold dilution with deionised water. The proposed method was successfully applied to the determination of thiocyanate in various human serum, saliva and urine samples.
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)01238-3