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High-level expression of canine parvovirus VP2 using Bombyx mori nucleopolyhedrovirus vector
For the potential use as recombinant vaccine, canine parvovirus (CPV) major capsid protein VP2 was expressed using Bombyx mori nucleopolyhedrovirus (BmNPV) vector. CPV VP2 gene was introduced into polyhedrin-based BmNPV transfer vector pBmKSK3, and recombinant virus BmK1-Parvo was prepared. When ant...
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| Published in: | Archives of virology 2000-01, Vol.145 (1), p.171-177 |
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| Main Authors: | , , , , , , , , |
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| container_end_page | 177 |
| container_issue | 1 |
| container_start_page | 171 |
| container_title | Archives of virology |
| container_volume | 145 |
| creator | CHOI, J. Y WOO, S. D LEE, H. K HONG, H. K JE, Y. H PARK, J. H SONG, J. Y AN, S. H KANG, S. K |
| description | For the potential use as recombinant vaccine, canine parvovirus (CPV) major capsid protein VP2 was expressed using Bombyx mori nucleopolyhedrovirus (BmNPV) vector. CPV VP2 gene was introduced into polyhedrin-based BmNPV transfer vector pBmKSK3, and recombinant virus BmK1-Parvo was prepared. When anti-CPV.VP2 monoclonal antibody was employed in immunofluorescence staining, an intense signal was observed within BmK1-Parvo-infected Bm5 cells but not within uninfected cells or cells infected with a wild-type BmNPV-K1. In hemagglutination assay, the expression level of VP2 were 3.2 x 10(3) HA units/ml from infected Bm5 cells, 2.1x 10(5) HA units/larvae from infected larval fat body, and 1.6x 10(6) HA units/ml from infected larval hemolymph. These results suggested that BmNPV vector system using B. mori larva as host could be applied to efficient mass-production of recombinant vaccines. |
| doi_str_mv | 10.1007/s007050050014 |
| format | article |
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Y ; WOO, S. D ; LEE, H. K ; HONG, H. K ; JE, Y. H ; PARK, J. H ; SONG, J. Y ; AN, S. H ; KANG, S. K</creator><creatorcontrib>CHOI, J. Y ; WOO, S. D ; LEE, H. K ; HONG, H. K ; JE, Y. H ; PARK, J. H ; SONG, J. Y ; AN, S. H ; KANG, S. K</creatorcontrib><description>For the potential use as recombinant vaccine, canine parvovirus (CPV) major capsid protein VP2 was expressed using Bombyx mori nucleopolyhedrovirus (BmNPV) vector. CPV VP2 gene was introduced into polyhedrin-based BmNPV transfer vector pBmKSK3, and recombinant virus BmK1-Parvo was prepared. When anti-CPV.VP2 monoclonal antibody was employed in immunofluorescence staining, an intense signal was observed within BmK1-Parvo-infected Bm5 cells but not within uninfected cells or cells infected with a wild-type BmNPV-K1. In hemagglutination assay, the expression level of VP2 were 3.2 x 10(3) HA units/ml from infected Bm5 cells, 2.1x 10(5) HA units/larvae from infected larval fat body, and 1.6x 10(6) HA units/ml from infected larval hemolymph. These results suggested that BmNPV vector system using B. mori larva as host could be applied to efficient mass-production of recombinant vaccines.</description><identifier>ISSN: 0304-8608</identifier><identifier>EISSN: 1432-8798</identifier><identifier>DOI: 10.1007/s007050050014</identifier><identifier>PMID: 10664415</identifier><language>eng</language><publisher>Wien: Springer</publisher><subject>Animals ; Biological and medical sciences ; Biotechnology ; Bombyx - virology ; Bombyx mori ; Canine parvovirus ; Capsid - biosynthesis ; Capsid - genetics ; Capsid Proteins ; Cells, Cultured ; DNA, Recombinant - genetics ; Dogs ; Fluorescent Antibody Technique, Indirect ; Fundamental and applied biological sciences. Psychology ; Genetic Vectors ; Health. Pharmaceutical industry ; Hemagglutination Tests ; Hemolymph - virology ; Industrial applications and implications. Economical aspects ; Nuclear polyhedrosis virus ; Nucleopolyhedrovirus - genetics ; Occlusion Body Matrix Proteins ; Parvovirus ; Parvovirus, Canine - genetics ; Parvovirus, Canine - metabolism ; Production of active biomolecules ; Promoter Regions, Genetic ; Recombinant Proteins - biosynthesis ; Vaccins ; Viral Proteins - genetics ; Viral Structural Proteins ; VP2 protein</subject><ispartof>Archives of virology, 2000-01, Vol.145 (1), p.171-177</ispartof><rights>2000 INIST-CNRS</rights><rights>Springer-Verlag/Wien 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-1150a84dc813381ac46e4f8151f12101d7abd1546147f3ff167899867d39594e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1278174$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10664415$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CHOI, J. Y</creatorcontrib><creatorcontrib>WOO, S. D</creatorcontrib><creatorcontrib>LEE, H. K</creatorcontrib><creatorcontrib>HONG, H. K</creatorcontrib><creatorcontrib>JE, Y. H</creatorcontrib><creatorcontrib>PARK, J. H</creatorcontrib><creatorcontrib>SONG, J. Y</creatorcontrib><creatorcontrib>AN, S. H</creatorcontrib><creatorcontrib>KANG, S. K</creatorcontrib><title>High-level expression of canine parvovirus VP2 using Bombyx mori nucleopolyhedrovirus vector</title><title>Archives of virology</title><addtitle>Arch Virol</addtitle><description>For the potential use as recombinant vaccine, canine parvovirus (CPV) major capsid protein VP2 was expressed using Bombyx mori nucleopolyhedrovirus (BmNPV) vector. CPV VP2 gene was introduced into polyhedrin-based BmNPV transfer vector pBmKSK3, and recombinant virus BmK1-Parvo was prepared. When anti-CPV.VP2 monoclonal antibody was employed in immunofluorescence staining, an intense signal was observed within BmK1-Parvo-infected Bm5 cells but not within uninfected cells or cells infected with a wild-type BmNPV-K1. In hemagglutination assay, the expression level of VP2 were 3.2 x 10(3) HA units/ml from infected Bm5 cells, 2.1x 10(5) HA units/larvae from infected larval fat body, and 1.6x 10(6) HA units/ml from infected larval hemolymph. These results suggested that BmNPV vector system using B. mori larva as host could be applied to efficient mass-production of recombinant vaccines.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Bombyx - virology</subject><subject>Bombyx mori</subject><subject>Canine parvovirus</subject><subject>Capsid - biosynthesis</subject><subject>Capsid - genetics</subject><subject>Capsid Proteins</subject><subject>Cells, Cultured</subject><subject>DNA, Recombinant - genetics</subject><subject>Dogs</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Vectors</subject><subject>Health. Pharmaceutical industry</subject><subject>Hemagglutination Tests</subject><subject>Hemolymph - virology</subject><subject>Industrial applications and implications. 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Y</au><au>WOO, S. D</au><au>LEE, H. K</au><au>HONG, H. K</au><au>JE, Y. H</au><au>PARK, J. H</au><au>SONG, J. Y</au><au>AN, S. H</au><au>KANG, S. K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-level expression of canine parvovirus VP2 using Bombyx mori nucleopolyhedrovirus vector</atitle><jtitle>Archives of virology</jtitle><addtitle>Arch Virol</addtitle><date>2000-01-01</date><risdate>2000</risdate><volume>145</volume><issue>1</issue><spage>171</spage><epage>177</epage><pages>171-177</pages><issn>0304-8608</issn><eissn>1432-8798</eissn><abstract>For the potential use as recombinant vaccine, canine parvovirus (CPV) major capsid protein VP2 was expressed using Bombyx mori nucleopolyhedrovirus (BmNPV) vector. CPV VP2 gene was introduced into polyhedrin-based BmNPV transfer vector pBmKSK3, and recombinant virus BmK1-Parvo was prepared. 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| subjects | Animals Biological and medical sciences Biotechnology Bombyx - virology Bombyx mori Canine parvovirus Capsid - biosynthesis Capsid - genetics Capsid Proteins Cells, Cultured DNA, Recombinant - genetics Dogs Fluorescent Antibody Technique, Indirect Fundamental and applied biological sciences. Psychology Genetic Vectors Health. Pharmaceutical industry Hemagglutination Tests Hemolymph - virology Industrial applications and implications. Economical aspects Nuclear polyhedrosis virus Nucleopolyhedrovirus - genetics Occlusion Body Matrix Proteins Parvovirus Parvovirus, Canine - genetics Parvovirus, Canine - metabolism Production of active biomolecules Promoter Regions, Genetic Recombinant Proteins - biosynthesis Vaccins Viral Proteins - genetics Viral Structural Proteins VP2 protein |
| title | High-level expression of canine parvovirus VP2 using Bombyx mori nucleopolyhedrovirus vector |
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