Olfactory Receptor Trafficking Involves Conserved Regulatory Steps

Olfactory receptors are difficult to functionally express in heterologous cells. They are typically retained in the endoplasmic reticulum of cells commonly used for functional expression studies and are only released to the plasma membrane in mature cells of the olfactory receptor neuron lineage. A...

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Bibliographic Details
Published in:The Journal of biological chemistry 2001-03, Vol.276 (10), p.7285-7290
Main Authors: Gimelbrant, Alexander A., Haley, Shannon L., McClintock, Timothy S.
Format: Article
Language:English
Subjects:
Animals
Caenorhabditis elegans Proteins
Cell Differentiation
Cell Line
Cell Membrane - metabolism
CHO Cells
Cricetinae
Endoplasmic Reticulum - metabolism
Endosomes - metabolism
Golgi Apparatus - metabolism
Immunohistochemistry
Microscopy, Confocal
Models, Biological
Olfactory Receptor Neurons - metabolism
Protein Transport
Rats
Receptors, Odorant - metabolism
Receptors, Odorant - physiology
Recombinant Proteins - metabolism
Transfection
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Summary:Olfactory receptors are difficult to functionally express in heterologous cells. They are typically retained in the endoplasmic reticulum of cells commonly used for functional expression studies and are only released to the plasma membrane in mature cells of the olfactory receptor neuron lineage. A recently developed olfactory cell line, odora, traffics olfactory receptors to the plasma membrane when differentiated. We found that undifferentiatedodora cells do not traffic olfactory receptors to their surface, even though they release the receptors to the Golgi apparatus and endosomes. This behavior differs from other cell lines tested thus far. Differentiated odora cells also properly traffic vomeronasal receptors of the VN1 type, which lack sequence similarity to olfactory receptors. ODR-4, a protein that is necessary for plasma membrane trafficking of a chemosensory receptor in nematodes, facilitates trafficking of rat olfactory receptor U131 inodora and Chinese hamster ovary cells. Olfactory receptor trafficking from the endoplasmic reticulum to the plasma membrane involves at least two steps whose regulation depends on the maturation state of cells in the olfactory receptor neuron lineage. These results also indicate that some components of the regulatory mechanism are conserved.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M005433200