Intensity dependence of cation kinetic energies from 2,5-dihydroxybenzoic acid near the infrared matrix-assisted laser desorption/ionization threshold

The mechanisms responsible for matrix‐assisted laser desorption/ionization (MALDI) are far from being well understood, particularly where infrared laser irradiation is used to initiate the process. We measured the emission yields and kinetic energy distributions of positive ions emitted from 2,5‐dih...

Full description

Saved in:
Bibliographic Details
Published in:Journal of mass spectrometry. 2001-05, Vol.36 (5), p.538-545
Main Authors: Ermer, D. R., Baltz-Knorr, M., Haglund Jr, R. F.
Format: Article
Language:English
Subjects:
Aminoacids, peptides. Hormones. Neuropeptides
Analytical, structural and metabolic biochemistry
Angiotensin II - chemistry
Biological and medical sciences
Cations
charge transfer
Chemistry
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Gentisates
Hydroxybenzoates - chemistry
infrared
Infrared Rays
ionization
Kinetics
Mass spectrometry
matrix-assisted laser desorption/ionization
Organic chemistry
Proteins
quadrupole
Reactivity and mechanisms
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The mechanisms responsible for matrix‐assisted laser desorption/ionization (MALDI) are far from being well understood, particularly where infrared laser irradiation is used to initiate the process. We measured the emission yields and kinetic energy distributions of positive ions emitted from 2,5‐dihydroxybenzoic acid loaded with angiotensin II in a standard MALDI preparation during irradiation with an infrared free‐electron laser tuned to 2.94 µm. As the laser intensity is scanned through the MALDI threshold, we see a marked change in the energy distributions of the matrix ion. Above threshold, the energy distributions of both analyte and matrix cations are constant over a broad range of laser intensities. This behavior does not appear to be consistent with any extant model of the MALDI mechanism. Copyright © 2001 John Wiley & Sons, Ltd.
ISSN:1076-5174
1096-9888
DOI:10.1002/jms.155