use of random amplified polymorphic DNA (RAPD) markers to identify strawberry varieties: a forensic application

The random amplified polymorphic DNA (RAPD) technique was applied to settle a lawsuit involving unauthorized commercialization of a patented strawberry variety of high economical relevance (‘Marmolada’®). Because of economical involvements, the molecular approach was added to the more traditional mo...

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Bibliographic Details
Published in:Molecular ecology 2000-02, Vol.9 (2), p.229-232
Main Authors: Congiu, L, Cella, R, Rossi, R, Bernacchia, G
Format: Article
Language:English
Subjects:
cultivar identification
DNA extraction
forensic
Fragaria ananassa
Fruit - classification
Fruit - genetics
Genetic Markers
laws and regulations
lawsuits
patented varieties
patented variety
Patents as Topic
Random Amplified Polymorphic DNA Technique
RAPD
strawberry
varieties
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Summary:The random amplified polymorphic DNA (RAPD) technique was applied to settle a lawsuit involving unauthorized commercialization of a patented strawberry variety of high economical relevance (‘Marmolada’®). Because of economical involvements, the molecular approach was added to the more traditional morphological examination in a double‐blind test. All plants belonging to the patented variety were unambiguously identified (13 plants among a total of 31 plants examined). The results were accepted as evidence in the court. This study confirms that the RAPD technique is especially suitable for identification of asexually reproduced plant varieties for forensic or agricultural purposes.
ISSN:0962-1083
1365-294X
DOI:10.1046/j.1365-294x.2000.00811.x