Expression of a highly conserved protein, p27BBP, during the progression of human colorectal cancer

The highly conserved protein p27BBP is a cytoplasmic interactor of integrin beta4 expressed in epithelia. p27BBP is found in two pools: one nuclear pool enriched in the perinucleolar region, and one cytoplasmic pool. Deletion of p27BBP in yeast is lethal as a result of loss of the ribosomal 60S subu...

Full description

Saved in:
Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 2000-02, Vol.60 (3), p.510-516
Main Authors: SANVITO, F, VIVOLI, F, GAMBINI, S, SANTAMBROGIO, G, CATENA, M, VIALE, E, VEGLIA, F, DONADINI, A, BIFFO, S, MARCHISIO, P. C
Format: Article
Language:English
Subjects:
Adenoma - chemistry
Animals
Antigens, CD - analysis
Biological and medical sciences
Carcinoma - chemistry
Carrier Proteins - analysis
Carrier Proteins - genetics
Colorectal Neoplasms - chemistry
Eukaryotic Initiation Factors
Gastroenterology. Liver. Pancreas. Abdomen
Gene Expression Regulation, Neoplastic
Humans
Integrin beta4
Intermediate Filament Proteins - analysis
Intermediate Filament Proteins - genetics
Intestinal Mucosa - chemistry
Medical sciences
Nucleolus Organizer Region - chemistry
Rabbits
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Transcription, Genetic
Tumors
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The highly conserved protein p27BBP is a cytoplasmic interactor of integrin beta4 expressed in epithelia. p27BBP is found in two pools: one nuclear pool enriched in the perinucleolar region, and one cytoplasmic pool. Deletion of p27BBP in yeast is lethal as a result of loss of the ribosomal 60S subunit. The aim of this study was to investigate the distribution of p27BBP in gut epithelium and its behavior during progression of human colorectal carcinomas. Results indicated that p27BBP is high in rapidly cycling cells and decreased in villous cells committed to apoptotic cell death. In dysplastic adenomas and carcinomas, p27BBP displayed a large increase of its nucleolar component that was superimposable to argyrophylic nucleolar organizing region-associated proteins and was associated with the nuclear matrix. Western blotting confirmed increased p27BBP in dysplastic adenomas and in carcinomas. In particular, p27BBP increased progressively from adenomas to carcinomas and, in the latter, was related to the tumor stage. The overexpression of p27BBP corresponded to mRNA up-regulation in carcinomas, supporting the idea of transcriptional or post-transcriptional regulation of its expression. Results suggested that p27BBP alterations are an early event in the transition from benign to malignant colorectal phenotypes and provide a novel tool in surgical pathology.
ISSN:0008-5472
1538-7445