beta subunit heterogeneity of L-type Ca(2+) channels in smooth muscle tissues

Various beta subunit isoforms stabilize different gating properties of voltage-gated L-type Ca(2+) channels. We therefore investigated the expression of Ca(2+) channel beta subunit isoforms in different smooth muscle types on the protein level by immunoblotting and immunoprecipitation employing beta...

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Bibliographic Details
Published in:FEBS letters 2000-02, Vol.467 (1), p.65-69
Main Authors: Reimer, D, Huber, I G, Garcia, M L, Haase, H, Striessnig, J
Format: Article
Language:English
Subjects:
Animals
Antibodies - immunology
Aorta
Blotting, Western
Calcium Channel Blockers - metabolism
Calcium Channels, L-Type - analysis
Calcium Channels, L-Type - chemistry
Calcium Channels, L-Type - immunology
Calcium Channels, L-Type - metabolism
Cattle
Cell Membrane - metabolism
Cerebral Cortex - chemistry
Female
Isradipine - metabolism
Molecular Weight
Muscle, Smooth - chemistry
Muscle, Smooth - immunology
Muscle, Smooth, Vascular - chemistry
Muscle, Smooth, Vascular - immunology
Myocardium - chemistry
Myometrium - chemistry
Myometrium - cytology
Myometrium - immunology
Organ Specificity
Precipitin Tests
Protein Isoforms - analysis
Protein Isoforms - chemistry
Protein Isoforms - immunology
Protein Isoforms - metabolism
Rabbits
Swine
Trachea - chemistry
Trachea - immunology
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Summary:Various beta subunit isoforms stabilize different gating properties of voltage-gated L-type Ca(2+) channels. We therefore investigated the expression of Ca(2+) channel beta subunit isoforms in different smooth muscle types on the protein level by immunoblotting and immunoprecipitation employing beta subunit-selective sequence-directed antibodies. From the four known beta subunit isoforms only beta2 and beta3 were detected in porcine uterus, bovine trachea and bovine aorta membranes. Multiple immunoreactive beta2 bands were detected in a tissue-selective manner indicating structural heterogeneity of beta2. Immunoprecipitation of (+)-[(3)H]isradipine-prelabeled channels revealed that beta2 and beta3 participate in Ca(2+) channel formation in uterus and trachea, and beta3 in aortic smooth muscle. We conclude that beta2 and beta3 subunits form L-type Ca(2+) channels in smooth muscle tissues. This subunit heterogeneity may be important to fine-tune channel function.
ISSN:0014-5793
DOI:10.1016/S0014-5793(00)01124-8