General expression of RoTat 1.2 variable antigen type in Trypanosoma evansi isolates from different origin

The variable surface glycoprotein of Trypanosoma evansi RoTat 1.2 variable antigen type (VAT) is used as an antigen in different antibody detection assays for T. evansi. To obtain more information on the predominant character of RoTat 1.2 and its diagnostic potential in antibody detection tests, we...

Full description

Saved in:
Bibliographic Details
Published in:Veterinary parasitology 2001-06, Vol.97 (3), p.185-191
Main Authors: Verloo, D., Magnus, E., Büscher, P.
Format: Article
Language:English
Subjects:
Agglutination Tests - veterinary
Animals
Antibodies, Protozoan - analysis
Antigens, Protozoan - biosynthesis
Antigens, Surface - biosynthesis
Brazil
Diagnosis-protozoa
Enzyme-Linked Immunosorbent Assay - veterinary
Equidae
Experimental
Fluorescent Antibody Technique - veterinary
Goats
Mice
Parasitemia - immunology
Parasitemia - veterinary
Philippines
Protozoan Proteins
Rabbit
Rabbits
Serology
Trypanosoma - classification
Trypanosoma - immunology
Trypanosoma evansi
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The variable surface glycoprotein of Trypanosoma evansi RoTat 1.2 variable antigen type (VAT) is used as an antigen in different antibody detection assays for T. evansi. To obtain more information on the predominant character of RoTat 1.2 and its diagnostic potential in antibody detection tests, we checked its expression in 10 different T. evansi stocks and clones from different parts of the world. Cryostabilates were injected into mice and the trypanosomes of the first peak parasitaemia were screened for the presence of RoTat 1.2 by VAT specific immunofluorescence. To monitor the appearance of RoTat 1.2 specific antibodies during infection, rabbits were infected and serologically tested at different time intervals with VAT specific immune trypanolysis, CATT/ T. evansi, LATEX/ T. evansi and ELISA/ T. evansi. Test results confirm the predominant character of RoTat 1.2.
ISSN:0304-4017
1873-2550
DOI:10.1016/S0304-4017(01)00412-5