Identification of a Novel LIM Domain Gene, LMCD1, and Chromosomal Localization in Human and Mouse

A new human LIM domain gene LMCD1 was identified from RT-PCR products, and partial sequencing of two expressed sequence tag clones flanking the assembled cDNA contig revealed a 1743-bp full-length cDNA with 1098-bp open reading frame. The coding sequence of the putative protein is 57.6% identical to...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 2000-01, Vol.63 (1), p.69-74
Main Authors: Bespalova, Irina N., Burmeister, Margit
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Biological and medical sciences
Blotting, Northern
Blotting, Southern
Carrier Proteins - genetics
Carrier Proteins - metabolism
chromosome 3
chromosome 6
Chromosome Mapping
Chromosomes, Human, Pair 3 - genetics
Classical genetics, quantitative genetics, hybrids
Co-Repressor Proteins
Embryo, Mammalian
Expressed Sequence Tags
Fundamental and applied biological sciences. Psychology
Genes. Genome
Genetics of eukaryotes. Biological and molecular evolution
Homeodomain Proteins - genetics
Homeodomain Proteins - metabolism
Human
Humans
Hybrid Cells
LIM Domain Proteins
LMCD1 gene
Mice
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Organ Specificity
radiation hybrid
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
Tes1 gene
Zinc Fingers
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Summary:A new human LIM domain gene LMCD1 was identified from RT-PCR products, and partial sequencing of two expressed sequence tag clones flanking the assembled cDNA contig revealed a 1743-bp full-length cDNA with 1098-bp open reading frame. The coding sequence of the putative protein is 57.6% identical to murine testin 1 gene (Tes1), whereas the predicted 365-amino-acid protein is 49.5% identical to the mouse Tes1 protein. The predicted LMCD1 protein contains a novel cysteine-rich domain residing in the amino-terminal region and two LIM domains with regular spacing in the carboxy-terminal region. Northern blot analysis indicated expression of the 1.7-kb transcript in many tissues, with highest abundance in skeletal muscle. Radiation hybrid mapping localized human LMCD1 to the telomeric region of chromosome 3p, and genetic mapping assigned the mouse Lmcd1 locus to the central region of chromosome 6.
ISSN:0888-7543
1089-8646
DOI:10.1006/geno.1999.6049