Vaxfectin enhances antigen specific antibody titers and maintains Th1 type immune responses to plasmid DNA immunization

Antigen specific immune responses were characterized after intramuscular immunization of BALB/c mice with 5 antigen encoding plasmid DNAs (pDNAs) complexed with Vaxfectin, a cationic lipid formulation. Vaxfectin increased IgG titers for all of the antigens with no effect on the CTL responses to the...

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Bibliographic Details
Published in:Vaccine 2001-06, Vol.19 (27), p.3778-3786
Main Authors: Reyes, Luane, Hartikka, Jukka, Bozoukova, Vesselina, Sukhu, Loretta, Nishioka, Walter, Singh, Gita, Ferrari, Marilyn, Enas, Joel, Wheeler, Carl J, Manthorpe, Marston, Wloch, Mary K
Format: Article
Language:English
Subjects:
Adjuvant
Adjuvants, Immunologic - administration & dosage
Animals
Antibody Specificity - immunology
Biological and medical sciences
Cationic lipid
Cytokines - biosynthesis
DNA vaccine
Drug Carriers
Epidemiology. Vaccinations
Ethanolamines
Female
Fundamental and applied biological sciences. Psychology
g-Interferon
General aspects
Immunity, Cellular - immunology
Immunoglobulin G - biosynthesis
immunoglobulin G1
immunoglobulin G2
Infectious diseases
Injections, Intramuscular
Interleukin-6 - deficiency
Interleukin-6 - immunology
Medical sciences
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Mutant Strains
Microbiology
Myristic Acids
Plasmids - administration & dosage
Plasmids - immunology
Th1 Cells - immunology
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Virology
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Summary:Antigen specific immune responses were characterized after intramuscular immunization of BALB/c mice with 5 antigen encoding plasmid DNAs (pDNAs) complexed with Vaxfectin, a cationic lipid formulation. Vaxfectin increased IgG titers for all of the antigens with no effect on the CTL responses to the 2 antigens for which CTL assays were performed. Both antigen specific IgG1 and IgG2a were increased, although IgG2a remained greater than IgG1. Furthermore, Vaxfectin had no effect on IFN-γ or IL-4 production by splenocytes re-stimulated with antigen, suggesting that the Th1 type responses typical of intramuscular pDNA immunization were not altered. Studies with IL-6 −/− mice suggest that the antibody enhancement is IL-6 dependent and results in a correlative increase in antigen specific antibody secreting cells.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(01)00090-1