Direct Interaction between Emerin and Lamin A

Emerin is the protein of the inner nuclear membrane that is affected by mutation in X-linked Emery-Dreifuss muscular dystrophy. The autosomal dominant form of the disease is caused by mutations in the lamin A/C gene. Several lines of circumstantial evidence have suggested an interaction of emerin wi...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2000-01, Vol.267 (3), p.709-714
Main Authors: Clements, L., Manilal, S., Love, D.R., Morris, G.E.
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal
BIAcore
Binding Sites
Cloning, Molecular
emerin
Emery-Dreifuss muscular dystrophy
Humans
lamin A/C gene
Lamin Type A
laminae
Lamins
Membrane Proteins - chemistry
Muscular Dystrophy, Emery-Dreifuss
nuclear lamina
Nuclear Proteins - chemistry
Peptide Fragments - chemistry
Recombinant Proteins - chemistry
Thymopoietins - chemistry
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Summary:Emerin is the protein of the inner nuclear membrane that is affected by mutation in X-linked Emery-Dreifuss muscular dystrophy. The autosomal dominant form of the disease is caused by mutations in the lamin A/C gene. Several lines of circumstantial evidence have suggested an interaction of emerin with lamins in the nuclear lamina but direct interaction between the two proteins has not yet been demonstrated. We now demonstrate direct interaction between recombinant emerin and lamin A molecules using biomolecular interaction analysis (BIA) and monoclonal antibodies. An emerin–lamin A interaction system may be related in function to the LAP2-lamin B system at the inner nuclear rim.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1999.2023