A rapid reversed phase high performance liquid chromatographic method for determination of etoposide (VP-16) in human plasma

A rapid, simple and sensitive isocratic High Performance Liquid Chromatography (HPLC) method was developed to measure the concentration of etoposide in plasma samples with UV detection at 220 nm. The method uses a Bondapac C18 column at 60 degrees C. The mobile phase consists of Methanol: water (45:...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2001-06, Vol.25 (3-4), p.353-356
Main Authors: SHIRAZI, F. H, BAHRAMI, G, STEWART, D. J, TOMIAK, E, DELORME, F, NOEL, D, GOEL, R
Format: Article
Language:English
Subjects:
Analysis
Antineoplastic agents
Biological and medical sciences
Chromatography, High Pressure Liquid
Etoposide - blood
General aspects
General pharmacology
Humans
Medical sciences
Pharmacology. Drug treatments
Reproducibility of Results
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Summary:A rapid, simple and sensitive isocratic High Performance Liquid Chromatography (HPLC) method was developed to measure the concentration of etoposide in plasma samples with UV detection at 220 nm. The method uses a Bondapac C18 column at 60 degrees C. The mobile phase consists of Methanol: water (45:55 v/v) at a flow rate of 2.8 ml/min. Phenacetin was used as an internal standard. The plasma samples were extracted using ether with the organic layer evaporated under nitrogen. The residue was dissolved in 200 microl methanol with 20 microl injected into the HPLC column. The extraction method showed a recovery of 91.5+/-3% for etoposide. In this system, the retention time of phenacetin and etoposide were 3.3 and 4.4 min, respectively. The limit of detection of etoposide in plasma is 20 ng/ml and the limit of quantitation is 40 ng/ml. This analytical method has very good reproducibility (8.1% between-day variability at a concentration of 50 ng/ml). It is a fast, sensitive and economic method applicable for clinical and pharmacokinetic studies.
ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(00)00520-3