Adapter proteins SLP-76 and BLNK both are expressed by murine macrophages and are linked to signaling via Fcgamma receptors I and II/III

The SLP-76 (Src homology 2 domain-containing leukocyte protein of 76 kDa) adapter protein is expressed in T cells and myeloid cells, whereas its homologue BLNK (B cell linker protein) is expressed in B cells. SLP-76 and BLNK link immunoreceptor tyrosine-based activation motif-containing receptors to...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2000-02, Vol.97 (4), p.1725-1730
Main Authors: Bonilla, F A, Fujita, R M, Pivniouk, V I, Chan, A C, Geha, R S
Format: Article
Language:English
Subjects:
Adaptor Proteins, Signal Transducing
Animals
B-Lymphocytes - metabolism
Bone Marrow - metabolism
Carrier Proteins - metabolism
Cross-Linking Reagents
Flow Cytometry
Immunology
Leukocytes
Macrophages - metabolism
Mice
Phagocytosis
Phosphoproteins - deficiency
Phosphoproteins - metabolism
Phosphorylation
Precipitin Tests
Proteins
Receptors, IgG - metabolism
Signal Transduction
T-Lymphocytes - metabolism
Tyrosine - metabolism
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Summary:The SLP-76 (Src homology 2 domain-containing leukocyte protein of 76 kDa) adapter protein is expressed in T cells and myeloid cells, whereas its homologue BLNK (B cell linker protein) is expressed in B cells. SLP-76 and BLNK link immunoreceptor tyrosine-based activation motif-containing receptors to signaling molecules that include phospholipase C-gamma, mitogen-activated protein kinases, and the GTPases Ras and Rho. SLP-76 plays a critical role in T cell receptor, FcvarepsilonRI and gpVI collagen receptor signaling, and participates in signaling via FcgammaR and killer cell inhibitory receptors. BLNK plays a critical role in B cell receptor signaling. We show that murine bone marrow-derived macrophages express both SLP-76 and BLNK. Selective ligation of FcgammaRI and FcgammaRII/III resulted in tyrosine phosphorylation of both SLP-76 and BLNK. SLP-76(-/-) bone marrow-derived macrophages display FcgammaR-mediated tyrosine phosphorylation of Syk, phospholipase C-gamma2, and extracellular signal regulated kinases 1 and 2, and normal FcgammaR-dependent phagocytosis. These data suggest that both SLP-76 and BLNK are coupled to FcgammaR signaling in murine macrophages.
ISSN:0027-8424
1091-6490