The effect of reactive oxygen species generated from the mitochondrial electron transport chain on the cytochrome c oxidase activity and on the cardiolipin content in bovine heart submitochondrial particles

The effect of reactive oxygen species (ROS), produced by the mitochondrial respiratory chain, on the activity of cytochrome c oxidase and on the cardiolipin content in bovine heart submitochondrial particles (SMP) was studied. ROS were produced by treatment of succinate-respiring SMP with antimycin...

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Bibliographic Details
Published in:FEBS letters 2000-01, Vol.466 (2), p.323-326
Main Authors: Paradies, Giuseppe, Petrosillo, Giuseppe, Pistolese, Marilva, Ruggiero, Francesca Maria
Format: Article
Language:English
Subjects:
Animals
Bovine heart submitochondrial particle
Cardiolipin
Cardiolipins - metabolism
Cattle
Cytochrome c oxidase
Electron Transport
Electron Transport Complex IV - metabolism
Mitochondria, Heart - drug effects
Mitochondria, Heart - enzymology
Mitochondria, Heart - metabolism
Reactive Oxygen Species
Submitochondrial Particles - drug effects
Submitochondrial Particles - enzymology
Submitochondrial Particles - metabolism
Superoxide Dismutase - pharmacology
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Summary:The effect of reactive oxygen species (ROS), produced by the mitochondrial respiratory chain, on the activity of cytochrome c oxidase and on the cardiolipin content in bovine heart submitochondrial particles (SMP) was studied. ROS were produced by treatment of succinate-respiring SMP with antimycin A. This treatment resulted in a large production of superoxide anion, measured by epinephrine method, which was blocked by superoxide dismutase (SOD). Exposure of SMP to mitochondrial mediated ROS generation, led to a marked loss of cytochrome c oxidase activity and to a parallel loss of cardiolipin content. Both these effects were completely abolished by SOD+catalase. Added cardiolipin was able to almost completely restore the ROS-induced loss of cytochrome c oxidase activity. No restoration was obtained with peroxidized cardiolipin. These results demonstrate that mitochondrial mediated ROS generation affects the activity of cytochrome c oxidase via peroxidation of cardiolipin which is needed for the optimal functioning of this enzyme complex. These results may prove useful in probing molecular mechanism of ROS-induced peroxidative damage to mitochondria which have been proposed to contribute to aging, ischemia/reperfusion and chronic degenerative diseases.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(00)01082-6