Subcommissural organ–Reissner's fiber complex of the teleost Clarias batrachus responds to GABA treatment

• Published in: Brain research 2000-01, Vol.852 (2), p.335-343
• Main Authors: Saha, Subhash G, Jain, Mukul R, Subhedar, Nishikant
• Format: Article
• Language: English
• Subjects: Animals; Antibodies; Bicuculline - pharmacology; Biological and medical sciences; Brain Chemistry - physiology; Calcitonin - analysis; Calcitonin - immunology; Calcitonin-like immunoreactivity; Catfishes - physiology; Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges; Female; Freshwater; Fundamental and applied biological sciences. Psychology; GABA; GABA Antagonists - pharmacology; gamma-Aminobutyric Acid - pharmacology; Immunocytochemistry; Immunohistochemistry; Male; Subcommissural Organ - chemistry; Subcommissural Organ - drug effects; Subcommissural Organ - physiology; Subcommissural organ–Reissner's fiber complex; Teleost; Vertebrates: nervous system and sense organs; γ-Aminobutyric acid
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/S0006-8993(99)02144-7

Subcommissural organ (SCO) is a highly specialized ependymal gland located in the roof of the third ventricle. The secretory products of the SCO, which condense to form Reissner's fiber (RF), were recently found to cross-react with the anti-calcitonin antibody. To understand the mechanisms regulating the formation of the RF and the possible function of these discrete structures, we studied the response of the SCO–RF complex to intracranially administered GABA, using immunocytochemical labeling with anti-calcitonin antibody. Although the SCO–RF complex of control fish was intensely immunostained, 1 h after GABA treatment, the ependymal cells revealed partial loss of immunoreactivity; the RF showed occasional loss of immunoreactivity with its diameter increased by about 56% of the control value. Following 2 h of GABA treatment, the SCO revealed dramatic loss of calcitonin-like immunoreactivity from the ependymal cells. The RF showed a dual response in this group, while in some segments the RF appeared conspicuously thick, elsewhere it appeared thin. The mean diameter was, however, not significantly different from the normal. Following 4 h of GABA treatment, while calcitonin-like immunoreactive material made its reappearance in the SCO, the RF diameter was uniformly reduced to about 35% of the control value. The responses by the RF as well as the SCO to intracranially administered GABA were blocked by pretreatment with bicuculline, a GABA A receptor antagonist. The results suggest that GABA, acting via GABA A receptors, may trigger the release of secretory material from the SCO and induce histomorphological changes in the RF indicative of discharge of stored material.