Structure elucidation of glycoprotein glycans and of polysaccharides by NMR spectroscopy

The applicability of 1H-NMR spectroscopy for the determination of the primary and tertiary structure of carbohydrate-containing molecules is demonstrated. For classes of known compounds the characterization can be based on chemical shifts observed in 1D NMR spectra with or without the aid of a compu...

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Bibliographic Details
Published in:Journal of biotechnology 2000-01, Vol.77 (1), p.115-122
Main Authors: Leeflang, B.R, Faber, E.J, Erbel, P, Vliegenthart, J.F.G
Format: Article
Language:English
Subjects:
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Bacterial Proteins - analysis
Bacterial Proteins - chemistry
Biological and medical sciences
Carbohydrate Conformation
Carbohydrate Sequence
Chorionic Gonadotropin - analysis
Chorionic Gonadotropin - chemistry
Exopolysaccharides
Fundamental and applied biological sciences. Psychology
glycans
Glycoproteins
Glycoproteins - analysis
Glycoproteins - chemistry
Hormones
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Molecular structure
NMR spectroscopy
Nuclear magnetic resonance spectroscopy
Polysaccharides
Polysaccharides, Bacterial - analysis
Polysaccharides, Bacterial - chemistry
Protein Structure, Tertiary
Proteins
Protons
Software
Structure determination
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Summary:The applicability of 1H-NMR spectroscopy for the determination of the primary and tertiary structure of carbohydrate-containing molecules is demonstrated. For classes of known compounds the characterization can be based on chemical shifts observed in 1D NMR spectra with or without the aid of a computer database. For more complex structure determinations 2D NMR techniques are required. Here the application of 2D NMR is demonstrated for the primary structure determination of two bacterial exopolysaccharides, for the spatial structure determination of a disaccharide and a glycoprotein hormone.
ISSN:0168-1656
1873-4863
DOI:10.1016/S0168-1656(99)00212-6