The enzymatic transformation of water-insoluble reactants in nonaqueous solvents. Conversion of cholesterol to cholest-4-ene-3-one by a Nocardia sp. Reprinted from Biotechnology and Bioengineering, Vol. XVII, Pages 815-826 (1975)

The rapid conversion of cholesterol to cholestenone by Nocardia in the presence of high proportions of water-immiscible solvent has been demonstrated. At high agitator speeds, the reaction rate was not limited by the rates of transfer of oxygen or cholesterol to the microorganisms. Using 100 g of th...

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Bibliographic Details
Published in:Biotechnology and bioengineering 2000-03, Vol.67 (6), p.714-719
Main Authors: Buckland, B C, Dunnill, P, Lilly, M D
Format: Article
Language:English
Subjects:
Buckland
Cholesterol - analogs & derivatives
Cholesterol - history
Cholesterol - metabolism
Dunnill
History, 20th Century
Lilly
Nocardia - metabolism
Solubility
Water - chemistry
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Summary:The rapid conversion of cholesterol to cholestenone by Nocardia in the presence of high proportions of water-immiscible solvent has been demonstrated. At high agitator speeds, the reaction rate was not limited by the rates of transfer of oxygen or cholesterol to the microorganisms. Using 100 g of thawed cells in 200 ml of carbon tetrachloride containing 16% (w/v) cholesterol, at 20 degrees C cholestenone was formed at 7 g/hr. Cells could be separated easily from the organic solvent and reused. After 7 runs (69 hr) the reaction rate had fallen only to half the value for the first run.
ISSN:0006-3592