Canine echinococcosis: an alternative for surveillance epidemiology

The essential activities for programmes of cystic echinococcosis control are the census of all dogs from the program and identification of parasitised animals. Currently, in South America evaluations and epidemiological surveillance are based on the administration of arecoline hydrobromide. This met...

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Bibliographic Details
Published in:Veterinary parasitology 2000-02, Vol.88 (1), p.131-134
Main Authors: Guarnera, E.A., Santillan, G., Botinelli, R., Franco, A.
Format: Article
Language:English
Subjects:
Animals
antigen detection
antigens
Antigens, Helminth - analysis
Arecoline - therapeutic use
Blotting, Western - veterinary
Canine dry-faeces
Cholinergic Agonists - therapeutic use
Copro-Western blot
Coproantigen
coproantigens
Diagnosis
Dog Diseases - epidemiology
Dog Diseases - parasitology
Dog Diseases - prevention & control
dog feces
Dogs
echinococcosis
Echinococcosis - epidemiology
Echinococcosis - prevention & control
Echinococcosis - veterinary
Echinococcus - growth & development
Echinococcus - immunology
Echinococcus granulosus
Electrophoresis, Polyacrylamide Gel - veterinary
enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - veterinary
epidemiological studies
Feces - parasitology
Sensitivity and Specificity
Sentinel Surveillance - veterinary
serodiagnosis
South America - epidemiology
Surveillance epidemiology
Western blotting
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Summary:The essential activities for programmes of cystic echinococcosis control are the census of all dogs from the program and identification of parasitised animals. Currently, in South America evaluations and epidemiological surveillance are based on the administration of arecoline hydrobromide. This method has the disadvantage of increasing environmental pollution and risk for operators and owners of treated dogs. A genus-specific ELISA capture method has been employed for recently issued faeces and the confirmation of positive examination was performed by dog autopsies. Our work presents an alternative method based on collection of dry field-dispersed faeces, followed by serological diagnosis by Copro-ELISA and confirmation by Copro-Western blot. If Copro-ELISA were used to define positive samples of dry faeces, the Copro-Western blot assay would provide 70% sensitivity and 100% specificity. Global efficiency of the system using dry faeces would reach 76%, allowing epidemilogical surveillance to be oriented to analysis of surface units instead of dog as measurement unit.
ISSN:0304-4017
1873-2550
DOI:10.1016/S0304-4017(99)00188-0