Characterisation of the loci encoding the glutamic acid and alanine rich protein of Trypanosoma congolense

We have characterised the organisation of genes encoding the glutamate and alanine rich protein (GARP) surface coat of the procyclic and epimastigote stages of Trypanosoma congolense in the tsetse fly. The GARP genes are arranged at two, possibly physically linked, loci, one of which exhibits alleli...

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Bibliographic Details
Published in:Molecular and biochemical parasitology 2000-02, Vol.105 (2), p.281-290
Main Authors: Rangarajan, Desikan, Harvey, Timothy I., Barry, J.David
Format: Article
Language:English
Subjects:
alanine
alleles
Amino Acid Sequence
amino acid sequences
Animals
Blotting, Southern
Chromosome Mapping
developmental stages
epimastigotes
GARP
GARP gene
garp genes
genbank/af173983
gene location
Genes, Protozoan
genomics
glutamate and alanine rich protein
glutamic acid
Glutamic acid and alanine rich protein
loci
membrane proteins
Molecular Sequence Data
nucleotide sequences
Polymerase Chain Reaction
procyclic stage
Procyclic/epimastigote surface coat
Protozoan Proteins - chemistry
Protozoan Proteins - genetics
Protozoan Proteins - metabolism
Sequence Alignment
Sequence Analysis, DNA
structural genes
transcription (genetics)
Transcription, Genetic
Trypanosoma congolense
Trypanosoma congolense - genetics
Trypanosoma congolense - growth & development
Trypanosoma congolense - metabolism
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Summary:We have characterised the organisation of genes encoding the glutamate and alanine rich protein (GARP) surface coat of the procyclic and epimastigote stages of Trypanosoma congolense in the tsetse fly. The GARP genes are arranged at two, possibly physically linked, loci, one of which exhibits allelic variation. One locus contains a single GARP gene, whilst both alleles of the other have a large tandem array of polycistronically transcribed GARP genes. Sequence analysis has revealed that there are very few coding differences between different GARP genes. A sequence related to the Trypanosoma brucei expression site associated gene 4 (encoding a transmembrane protein with a cytoplasmic adenylate cyclase domain) has been identified within a region at the downstream flank of one locus. There is no evidence that, within the single trypanosome, GARP genes are as diverse as the procyclin genes that encode a corresponding coat in T. brucei.
ISSN:0166-6851
1872-9428
DOI:10.1016/S0166-6851(99)00190-5