Structural characterisation of the exopolysaccharide produced by Streptococcus thermophilus EU20

Streptococcus thermophilus EU20 when grown on skimmed milk secretes a high-molecular-weight exopolysaccharide that is composed of glucose, galactose and rhamnose in a molar ratio of 2:3:2. Using chemical techniques and 1D and 2D-NMR spectroscopy ( 1H and 13C) the polysaccharide has been shown to pos...

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Bibliographic Details
Published in:Carbohydrate research 2001-04, Vol.331 (4), p.413-422
Main Authors: Marshall, Valerie M., Dunn, Helen, Elvin, Mark, McLay, Neil, Gu, Yucheng, Laws, Andrew P.
Format: Article
Language:English
Subjects:
Carbohydrate Sequence
Exopolysaccharide
Heptasaccharide repeating unit
Molecular Sequence Data
Nuclear Magnetic Resonance, Biomolecular
Oligosaccharides - chemistry
Polysaccharides, Bacterial - chemistry
Sequence Analysis
Streptococcus - chemistry
Streptococcus thermophilus
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Summary:Streptococcus thermophilus EU20 when grown on skimmed milk secretes a high-molecular-weight exopolysaccharide that is composed of glucose, galactose and rhamnose in a molar ratio of 2:3:2. Using chemical techniques and 1D and 2D-NMR spectroscopy ( 1H and 13C) the polysaccharide has been shown to possess a heptasaccharide repeating unit having the following structure: Treatment of the polysaccharide with mild acid (0.5 M TFA, 100 °C for 1 h) liberates two oligosaccharides; the components correspond to the repeating unit and a hexasaccharide equivalent to the repeating unit minus the terminal α- l-Rha p.
ISSN:0008-6215
1873-426X
DOI:10.1016/S0008-6215(01)00052-0