Extraction of iridoid glycosides and their determination by micellar electrokinetic capillary chromatography

Several methods for the extraction of two iridoid glycosides, catalpol and aucubin, from the plant matrix ( Veronica longifolia leaves) were compared. Pressurized hot water extraction and hot water extraction were the most efficient isolation techniques for both. Pressurized liquid extraction and ma...

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Bibliographic Details
Published in:Journal of Chromatography A 2000-01, Vol.868 (1), p.73-83
Main Authors: Suomi, Johanna, Sirén, Heli, Hartonen, Kari, Riekkola, Marja-Liisa
Format: Article
Language:English
Subjects:
Aucubin
Biological and medical sciences
Catalpol
Chromatography, Micellar Electrokinetic Capillary - methods
General pharmacology
Glucosides - isolation & purification
Glycosides
Hot Temperature
Iridoid Glucosides
Iridoid glycosides
Iridoids
Medical sciences
Monoterpenes
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Plant Leaves - chemistry
Pressure
Pyrans - isolation & purification
Solvents
Terpenes
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Summary:Several methods for the extraction of two iridoid glycosides, catalpol and aucubin, from the plant matrix ( Veronica longifolia leaves) were compared. Pressurized hot water extraction and hot water extraction were the most efficient isolation techniques for both. Pressurized liquid extraction and maceration with various organic solvents were also tested. Relative to the amounts extracted with hot water, ethanol extracted only 22% of catalpol and 25% of aucubin and pressurized hot water extracted 83% of catalpol and 92% of aucubin. The lowest relative standard deviations, 22% for catalpol and 8% for aucubin, were achieved with hot water extraction (13 repetitions), and the highest relative standard deviations, 76% for catalpol and 73% for aucubin, with pressurized liquid extraction (five repetitions). A fast capillary electrophoretic method was developed for the quantitative determination of catalpol and aucubin.
ISSN:0021-9673
DOI:10.1016/S0021-9673(99)01170-X