Sequence, protein expression and extracellular-regulated kinase association of the hyaladherin RHAMM (receptor for hyaluronan mediated motility) in PC12 cells

• Published in: Neuroscience letters 2001-06, Vol.306 (1), p.49-52
• Main Authors: Lynn, B.D, Li, Xinbo, Cattini, P.A, Nagy, J.I
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence - physiology; Animals; Base Sequence - genetics; Biological and medical sciences; Cell Compartmentation - genetics; Cell Differentiation - drug effects; Cell Differentiation - physiology; Cytoskeleton; DNA, Complementary - chemistry; DNA, Complementary - genetics; DNA, Complementary - isolation & purification; Extracellular Matrix Proteins - genetics; Extracellular Matrix Proteins - isolation & purification; Extracellular Matrix Proteins - metabolism; Extracellular regulated kinase signaling; extracellular signal-regulated kinase; Fibroblast Growth Factor 2 - pharmacology; Fundamental and applied biological sciences. Psychology; Gene Expression - physiology; Growth cone; Hyaluronan; Hyaluronan Receptors - genetics; Hyaluronan Receptors - isolation & purification; Hyaluronan Receptors - metabolism; hyaluronic acid; Immunohistochemistry; Isolated neuron and nerve. Neuroglia; Map kinase; MAP Kinase Signaling System - drug effects; MAP Kinase Signaling System - physiology; Mitogen-Activated Protein Kinases - drug effects; Mitogen-Activated Protein Kinases - metabolism; Nerve Growth Factor - pharmacology; PC12 Cells - cytology; PC12 Cells - drug effects; PC12 Cells - metabolism; Protein Isoforms - genetics; Protein Isoforms - isolation & purification; Protein Isoforms - metabolism; Rats; Receptor tyrosine kinase; RHAMM protein; RNA, Messenger - chemistry; RNA, Messenger - genetics; RNA, Messenger - isolation & purification; Vertebrates: nervous system and sense organs
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/S0304-3940(01)01870-5

Differentiation of PC12 cells by nerve growth factor (NGF) or fibroblast growth factor-2 (FGF2) is dependent on signaling mediated by extracellular regulated kinase (ERK). We investigated the involvement of receptor for hyaluronan mediated motility (RHAMM) in this signaling pathway. A single RHAMM 3.2 kb transcript was detected in PC12 RNA. Reverse transcriptase-polymerase chain reaction generated a 2141 bp cDNA that had identical sequence to rat brain RHAMM and showed no evidence of alternate splicing. Several RHAMM species were identified by Western blotting. Immunohistochemistry showed RHAMM localization to the cytoskeleton, neurites and growth cones. Following stimulation of PC12 cells with NGF or FGF2 RHAMM was co-immunoprecipitated by phosphorylation-specific anti-ERK antibodies, indicating a role for RHAMM in ERK signaling in PC12 cells.