Kalirin, a GDP/GTP Exchange Factor of the Dbl Family, Is Localized to Nerve, Muscle, and Endocrine Tissue During Embryonic Rat Development

Kalirin, a homologue of trio and UNC-73, has been previously demonstrated to cause cytoskeletal rearrangements, enhanced outgrowth of neuritic processes, and altered secretion. In the adult rat, kalirin is specifically localized to the central nervous system, with the main adult isoform, kalirin-7,...

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Bibliographic Details
Published in:The journal of histochemistry and cytochemistry 2001-07, Vol.49 (7), p.833-844
Main Authors: Hansel, Donna E, Quinones, Mercedes E, Ronnett, Gabriele V, Eipper, Betty A
Format: Article
Language:English
Subjects:
Animals
Blotting, Western
Brain - metabolism
Carrier Proteins
Embryonic and Fetal Development
Endocrine System - metabolism
Guanine Nucleotide Exchange Factors - metabolism
Immunohistochemistry
Muscle, Skeletal - metabolism
Nerve Tissue - metabolism
Neurons - metabolism
Olfactory Pathways - metabolism
Organ Specificity
Protein Isoforms - metabolism
Rats
Rats, Sprague-Dawley
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Summary:Kalirin, a homologue of trio and UNC-73, has been previously demonstrated to cause cytoskeletal rearrangements, enhanced outgrowth of neuritic processes, and altered secretion. In the adult rat, kalirin is specifically localized to the central nervous system, with the main adult isoform, kalirin-7, concentrated in neuronal postsynaptic densities. In this study we examined the expression of kalirin in rat tissue from embryonic Day 10 (E10) through E18, using an antibody that detects all known kalirin isoforms. Kalirin expression in the embryo was more widespread than in the adult, with localization of kalirin protein to both neuronal and non-neuronal tissue, such as muscle, lung, intestinal epithelium, and pancreas. In neurons, kalirin was localized both in cell bodies and axon processes; in muscle tissue, kalirin was highly localized to migrating myogenic cells and at muscle attachment sites. Western blotting analysis indicated that kalirin-7, the major adult isoform, was a minor component of embryonic kalirin; the main isoform expressed in the embryo was kalirin-9. This is the first identification of kalirin expression in embryonic tissue and the first demonstration of non-neuronal expression of kalirin.
ISSN:0022-1554
1551-5044
DOI:10.1177/002215540104900704