Relaxin-like factor (RLF) mRNA expression in the fallow deer

Employing RT- and RACE-PCR on RNA isolated from testicular tissue, we have cloned the coding cDNA sequence for the RLF, also known as Insl3, of the fallow deer. The RLF coding sequence consisted of 396 bp encoding a peptide of 131 amino acids and shared highest homology with bovine, sheep and goat R...

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Bibliographic Details
Published in:Molecular and cellular endocrinology 2000-01, Vol.159 (1), p.147-158
Main Authors: Hombach-Klonisch, Sabine, Kauffold, Johannes, Rautenberg, Tanja, Steger, Klaus, Tetens, Frank, Fischer, Bernd, Klonisch, Thomas
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Cattle
Cloning, Molecular
Deer
Deer - genetics
Deer - metabolism
DNA, Complementary - genetics
Female
Gene Expression
Genitalia, Female - metabolism
Goats
In Situ Hybridization
Insl3
Insulin
Male
MHC class I
Molecular Sequence Data
Ovary
Placenta
Pregnancy
Proteins - genetics
Relaxin-like factor
RLF
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sequence Homology, Amino Acid
Sheep
Species Specificity
Testis
Testis - metabolism
Tissue Distribution
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Summary:Employing RT- and RACE-PCR on RNA isolated from testicular tissue, we have cloned the coding cDNA sequence for the RLF, also known as Insl3, of the fallow deer. The RLF coding sequence consisted of 396 bp encoding a peptide of 131 amino acids and shared highest homology with bovine, sheep and goat RLF. Northern analysis revealed a single 0.9 kb transcript in the deer testis. There is only one RLF gene in the deer genome. Nonradioactive in situ hybridization revealed the Leydig cells to be the sole source for RLF mRNA in the deer testis. In the non-pregnant uterus, RLF transcripts were located in the luminal and glandular epithelium of the endometrium. Within the ovary of the pregnant doe, follicular theca interna cells and the corpus luteum expressed RLF transcripts. In uteroplacental tissues, luminal and glandular epithelium, fetal uninucleate and binucleate trophoblast cells (BNC) of the basic villous trophoblast layer expressed RLF mRNA. BNC located at the apical trophoblast layer or the tip of the fetal villus were devoid of RLF transcripts. Pseudostratified trophoblast cells at the base of fetal villi coexpressed RLF mRNA and immunoreactive MHC class Ib molecules.
ISSN:0303-7207
1872-8057
DOI:10.1016/S0303-7207(99)00190-2