Comparison of OPS imaging and conventional capillary microscopy to study the human microcirculation

Departments of 1  Anesthesiology, 2  Obstetrics, and 3  Surgery, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands Orthogonal polarization spectral (OPS) imaging is a new clinical technique for observation of the microcirculation of organ surfaces. For validation p...

Full description

Saved in:
Bibliographic Details
Published in:Journal of applied physiology (1985) 2001-07, Vol.91 (1), p.74-78
Main Authors: Mathura, Keshen R, Vollebregt, Karlijn C, Boer, Kees, De Graaff, Jurgen C, Ubbink, Dirk T, Ince, Can
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Blood Flow Velocity
Blood vessels and receptors
Capillaries - physiology
Circulatory system
Comparative analysis
Diagnostic Imaging
Erythrocytes - cytology
Erythrocytes - physiology
Fundamental and applied biological sciences. Psychology
Humans
Image Processing, Computer-Assisted
Male
Medical imaging
Microcirculation
Microscopy
Nails - blood supply
Reference Values
Studies
Vertebrates: cardiovascular system
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Departments of 1  Anesthesiology, 2  Obstetrics, and 3  Surgery, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands Orthogonal polarization spectral (OPS) imaging is a new clinical technique for observation of the microcirculation of organ surfaces. For validation purposes, we compared OPS images of the nailfold skin with those obtained from conventional capillary microscopy at rest and during venous occlusion in 10 male volunteers. These images were computer analyzed to provide red blood cell velocity and capillary diameters of the same nailfold capillaries at rest and during venous occlusion. Results showed that OPS images provided similar values for red blood cell velocity and capillary diameter as those obtained from capillary microscopy images. OPS imaging, however, provided significantly better image quality, as shown by comparison of image contrast between OPS imaging and capillary microscopy. This made image analysis better and easier to perform. It is anticipated, therefore, that OPS imaging will become a new and powerful technique in the study of the human microcirculation in vivo because it can be used on human internal organs. nailfold; intravital; validation; erythrocyte; contrast; orthogonal polarization spectral imaging
ISSN:8750-7587
1522-1601
DOI:10.1152/jappl.2001.91.1.74