Infrared-thermographic screening of the activity and enantioselectivity of enzymes

The infrared radiation caused by the heat of reaction of an enantioselective enzyme-catalyzed transformation can be detected by modern photovoltaic infrared (IR)-thermographic cameras equipped with focal-plane array detectors. Specifically, in the lipase-catalyzed enantioselective acylation of racem...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2001-05, Vol.55 (5), p.531-536
Main Authors: Reetz, M.T, Hermes, M, Becker, M.H
Format: Article
Language:English
Subjects:
1-phenylethanol
acylation
Biological and medical sciences
Biotechnology
cameras
Catalysis
detectors
enantiomers
Enzymes
Enzymes - metabolism
Fundamental and applied biological sciences. Psychology
heat
In Vitro Techniques
Infrared radiation
Infrared Rays
Kinetics
Methods. Procedures. Technologies
Others
Photovoltaics
screening
Stereoisomerism
Thermography - methods
Various methods and equipments
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Description
Summary:The infrared radiation caused by the heat of reaction of an enantioselective enzyme-catalyzed transformation can be detected by modern photovoltaic infrared (IR)-thermographic cameras equipped with focal-plane array detectors. Specifically, in the lipase-catalyzed enantioselective acylation of racemic 1-phenylethanol, the (R)- and (S)-substrates are allowed to react separately in the wells of microtiter plates, the (R)-alcohol showing hot spots in the IR-thermographic images. Thus, highly enantioselective enzymes can be identified at kinetic resolution.
ISSN:0175-7598
1432-0614
DOI:10.1007/s002530100597