yam8(+), a Schizosaccharomyces pombe gene, is a potential homologue of the Saccharomyces cerevisiae MID1 gene encoding a stretch-activated Ca(2+)-permeable channel

• Published in: Biochemical and biophysical research communications 2000-03, Vol.269 (1), p.265-269
• Main Authors: Tasaka, Y, Nakagawa, Y, Sato, C, Mino, M, Uozumi, N, Murata, N, Muto, S, Iida, H
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Base Sequence; Calcium Channels - chemistry; Calcium Channels - genetics; DNA Primers - genetics; DNA, Complementary - genetics; DNA, Fungal - genetics; Fungal Proteins - chemistry; Fungal Proteins - genetics; Gene Expression; Genes, Fungal; Genetic Complementation Test; Membrane Glycoproteins - chemistry; Membrane Glycoproteins - genetics; Molecular Sequence Data; Mutation; Phenotype; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins; Schizosaccharomyces - genetics; Schizosaccharomyces pombe Proteins; Sequence Homology, Amino Acid; Species Specificity
• ISSN: 0006-291X
• DOI: 10.1006/bbrc.2000.2278

The Saccharomyces cerevisiae MID1 gene encodes a stretch-activated Ca(2+)-permeable channel. In a protein database, we found a Schizosaccharomyces pombe gene whose predicted protein shows 26% identical and 62% similar to the Mid1 channel in amino acid sequence. cDNA derived from this gene, designated yam8(+), was isolated by reverse transcription-polymerase chain reaction (RT-PCR). Further analysis showed that the Yam8 protein consists of 486 amino acids and has 6 hydrophobic segments. The yam8(+) cDNA, placed under the S. cerevisiae TDH3 promoter, partially complemented the mating pheromone-induced death (mid) phenotype of the S. cerevisiae mid1 mutant. The expression of the yam8(+) cDNA in the mid1 mutant cells partially remediated the mid phenotype and resulted in a slight increase in Ca(2+) uptake activity. These findings suggest that Yam8 is a potential homologue of Mid1.