The H1 double-stranded RNA genome of Ustilago maydis virus-H1 encodes a polyprotein that contains structural motifs for capsid polypeptide, papain-like protease, and RNA-dependent RNA polymerase

The Ustilago maydis viral (UmV) genome consists of three distinct size groups of double-stranded RNA (dsRNA) segments: H (heavy), M (medium), and L (light). The H segments have been suggested to encode all essential viral proteins, but without any molecular evidences. As a preliminary step to unders...

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Bibliographic Details
Published in:Virus research 2001-08, Vol.76 (2), p.183-189
Main Authors: Kang, Jeong-Gu, Wu, Jae-Chang, Bruenn, Jeremy A., Park, Chung-Mo
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Capsid - genetics
DNA, Viral
Double-stranded RNA
Genome, Viral
Molecular Sequence Data
Papain - genetics
Peptides - genetics
Polyprotein
polyproteins
Polyproteins - genetics
RNA Replicase - genetics
RNA Viruses - genetics
RNA, Double-Stranded - analysis
RNA, Viral - analysis
RNA-dependent RNA polymerase
Ustilago - virology
Ustilago maydis virus
Ustilago maydis virus-H1 (UmV-H1)
Viral papain-like protease
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Summary:The Ustilago maydis viral (UmV) genome consists of three distinct size groups of double-stranded RNA (dsRNA) segments: H (heavy), M (medium), and L (light). The H segments have been suggested to encode all essential viral proteins, but without any molecular evidences. As a preliminary step to understand viral genomic organization and the molecular mechanism governing gene expression in UmV, we determined the complete nucleotide sequence of the H1 dsRNA genome in P1 viral killer subtype. The H1 dsRNA genome (designated UmV-H1) contained a single open reading frame that encodes a polyprotein of 1820 residues, which is predicted to be autocatalytically processed by a viral papain-like protease to generate viral proteins. The amino-terminal region is the capsid polypeptide with a predicted molecular mass of 79.9 kDa. The carboxy-terminal region is the RNA-dependent RNA polymerase (RDRP) that has a high sequence homology to those of the totiviruses. The H2 dsRNA also encodes a distinct RDRP, suggesting that UmV is a complex virus system like the Saccharomyces cerevisiae viruses ScV-L1 and -La.
ISSN:0168-1702
1872-7492
DOI:10.1016/S0168-1702(01)00250-7