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A tissue culture system to study respiratory ciliary epithelial adherence of selected swine mycoplasmas

An in vitro culture system for swine tracheal epithelial cells was developed to study the adherence of swine mycoplasmas. Swine tracheal epithelial cells were isolated by enzymatic digestion and cultured on microporous membranes. Growth medium was placed under the membrane support to create air–liqu...

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Published in:Veterinary microbiology 2000-02, Vol.71 (3), p.269-279
Main Authors: Young, Theresa F., Thacker, Eileen L., Erickson, Barbara Z., Ross, Richard F.
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description An in vitro culture system for swine tracheal epithelial cells was developed to study the adherence of swine mycoplasmas. Swine tracheal epithelial cells were isolated by enzymatic digestion and cultured on microporous membranes. Growth medium was placed under the membrane support to create air–liquid interface feeding resulting in the cells growing cilia and microvilli on the apical surface. Two strains of Mycoplasma hyopneumoniae (pathogenic strain 91-3 and non-pathogenic type strain J) and two strains of Mycoplasma flocculare (type strain Ms42 and field isolate 7160T) were used in this study. The morphology of the cultured tracheal cells was evaluated by transmission electron microscopy. Adherence of M. hyopneumoniae and M. flocculare and damage to the cilia were demonstrated using scanning electron microscopy. The pathogenic M. hyopneumoniae strain 91-3 adhered to cilia inducing obvious damage. The non-pathogenic M. hyopneumoniae strain J did not adhere to mature cilia. Both M. flocculare strains Ms42 and 7160T adhered to mature and budding cilia. No obvious ciliary damage was observed with strain Ms42. Minimal damage consisting of a slight tangling of the cilia occurred after adherence by strain 7160T. This model will enable us to further study the role of adherence of mycoplasmas on the pathogenesis of swine pneumonia.
doi_str_mv 10.1016/S0378-1135(99)00176-5
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Swine tracheal epithelial cells were isolated by enzymatic digestion and cultured on microporous membranes. Growth medium was placed under the membrane support to create air–liquid interface feeding resulting in the cells growing cilia and microvilli on the apical surface. Two strains of Mycoplasma hyopneumoniae (pathogenic strain 91-3 and non-pathogenic type strain J) and two strains of Mycoplasma flocculare (type strain Ms42 and field isolate 7160T) were used in this study. The morphology of the cultured tracheal cells was evaluated by transmission electron microscopy. Adherence of M. hyopneumoniae and M. flocculare and damage to the cilia were demonstrated using scanning electron microscopy. The pathogenic M. hyopneumoniae strain 91-3 adhered to cilia inducing obvious damage. The non-pathogenic M. hyopneumoniae strain J did not adhere to mature cilia. Both M. flocculare strains Ms42 and 7160T adhered to mature and budding cilia. No obvious ciliary damage was observed with strain Ms42. Minimal damage consisting of a slight tangling of the cilia occurred after adherence by strain 7160T. This model will enable us to further study the role of adherence of mycoplasmas on the pathogenesis of swine pneumonia.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>10703709</pmid><doi>10.1016/S0378-1135(99)00176-5</doi><tpages>11</tpages></addata></record>
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subjects Animals
Bacterial Adhesion - immunology
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Cell Count
Cilia - microbiology
Cilia - pathology
Cilia - ultrastructure
Ciliated epithelial cells
Culture Techniques - methods
Culture Techniques - veterinary
Epithelial Cells - microbiology
Epithelial Cells - ultrastructure
Fundamental and applied biological sciences. Psychology
Image Processing, Computer-Assisted
Microbiology
Microscopy, Electron - veterinary
Microscopy, Electron, Scanning - veterinary
Mycoplasma - immunology
Mycoplasma - pathogenicity
Mycoplasma flocculare
Mycoplasma hyopneumoniae
Pig-bacteria
Pneumonia of Swine, Mycoplasmal - immunology
Pneumonia of Swine, Mycoplasmal - pathology
Pneumonia of Swine, Mycoplasmal - veterinary
Swine
Swine Diseases - microbiology
Swine Diseases - pathology
Trachea - immunology
Trachea - microbiology
title A tissue culture system to study respiratory ciliary epithelial adherence of selected swine mycoplasmas
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