Leptin Regulation of Prepro-orexin and Orexin Receptor mRNA Levels in the Hypothalamus

The aim of this study was to determine the effects of leptin treatment on prepro-orexin and orexin receptor expression in the rat hypothalamus. Adult male rats, food-deprived for 48 and 72 h, were treated one time with vehicle or leptin (10 μg, icv). Prepro-orexin mRNA content was measured by semiqu...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2000-03, Vol.269 (1), p.41-45
Main Authors: López, Miguel, Seoane, Luisa, del Carmen García, María, Lago, Francisca, Casanueva, Felipe F., Señarís, Rosa, Diéguez, Carlos
Format: Article
Language:English
Subjects:
Animals
Eating - drug effects
Eating - genetics
Eating - physiology
fasting
Fasting - physiology
feeding
food deprivation
Food Deprivation - physiology
food intake
Gene Expression Regulation - drug effects
hypothalamus
Hypothalamus - drug effects
Hypothalamus - metabolism
Hypothalamus - physiology
In Situ Hybridization
Intracellular Signaling Peptides and Proteins
leptin
Leptin - pharmacology
Leptin - physiology
Male
messenger RNA
Neuropeptides
Northern blot
obesity
orexin
Orexin Receptors
Orexins
prepro-orexin
Protein Precursors - genetics
Protein Precursors - physiology
Rats
Rats, Sprague-Dawley
Receptors, G-Protein-Coupled
Receptors, Neuropeptide - genetics
Receptors, Neuropeptide - physiology
RNA, Messenger - genetics
RNA, Messenger - metabolism
RT-PCR
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Summary:The aim of this study was to determine the effects of leptin treatment on prepro-orexin and orexin receptor expression in the rat hypothalamus. Adult male rats, food-deprived for 48 and 72 h, were treated one time with vehicle or leptin (10 μg, icv). Prepro-orexin mRNA content was measured by semiquantitative RT-PCR, Northern blot, and in situ hybridization; orexin receptor 1 and 2 mRNA content was quantified by Northern blot and/or semiquantitative RT-PCR. Our results indicate that leptin inhibits a fasting-induced increase in prepro-orexin mRNA and orexin receptor 1 mRNA levels in the rat hypothalamus, while orexin receptor 2 mRNA levels were unchanged in all situations evaluated. These data provide direct evidence for an additional mechanism of adaptation of the hypothalamus to food deprivation and for a new effect of leptin in the regulation of food intake.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2000.2245