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Leptin Regulation of Prepro-orexin and Orexin Receptor mRNA Levels in the Hypothalamus
The aim of this study was to determine the effects of leptin treatment on prepro-orexin and orexin receptor expression in the rat hypothalamus. Adult male rats, food-deprived for 48 and 72 h, were treated one time with vehicle or leptin (10 μg, icv). Prepro-orexin mRNA content was measured by semiqu...
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| Published in: | Biochemical and biophysical research communications 2000-03, Vol.269 (1), p.41-45 |
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| Main Authors: | , , , , , , |
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| cites | cdi_FETCH-LOGICAL-c461t-9c981a2b1b74a84a1db995a4e1f3210076f8d62c5bbfbf9bd9faa2ae23dcdfab3 |
| container_end_page | 45 |
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| container_title | Biochemical and biophysical research communications |
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| creator | López, Miguel Seoane, Luisa del Carmen García, María Lago, Francisca Casanueva, Felipe F. Señarís, Rosa Diéguez, Carlos |
| description | The aim of this study was to determine the effects of leptin treatment on prepro-orexin and orexin receptor expression in the rat hypothalamus. Adult male rats, food-deprived for 48 and 72 h, were treated one time with vehicle or leptin (10 μg, icv). Prepro-orexin mRNA content was measured by semiquantitative RT-PCR, Northern blot, and in situ hybridization; orexin receptor 1 and 2 mRNA content was quantified by Northern blot and/or semiquantitative RT-PCR. Our results indicate that leptin inhibits a fasting-induced increase in prepro-orexin mRNA and orexin receptor 1 mRNA levels in the rat hypothalamus, while orexin receptor 2 mRNA levels were unchanged in all situations evaluated. These data provide direct evidence for an additional mechanism of adaptation of the hypothalamus to food deprivation and for a new effect of leptin in the regulation of food intake. |
| doi_str_mv | 10.1006/bbrc.2000.2245 |
| format | article |
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Adult male rats, food-deprived for 48 and 72 h, were treated one time with vehicle or leptin (10 μg, icv). Prepro-orexin mRNA content was measured by semiquantitative RT-PCR, Northern blot, and in situ hybridization; orexin receptor 1 and 2 mRNA content was quantified by Northern blot and/or semiquantitative RT-PCR. Our results indicate that leptin inhibits a fasting-induced increase in prepro-orexin mRNA and orexin receptor 1 mRNA levels in the rat hypothalamus, while orexin receptor 2 mRNA levels were unchanged in all situations evaluated. These data provide direct evidence for an additional mechanism of adaptation of the hypothalamus to food deprivation and for a new effect of leptin in the regulation of food intake.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.2000.2245</identifier><identifier>PMID: 10694474</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Eating - drug effects ; Eating - genetics ; Eating - physiology ; fasting ; Fasting - physiology ; feeding ; food deprivation ; Food Deprivation - physiology ; food intake ; Gene Expression Regulation - drug effects ; hypothalamus ; Hypothalamus - drug effects ; Hypothalamus - metabolism ; Hypothalamus - physiology ; In Situ Hybridization ; Intracellular Signaling Peptides and Proteins ; leptin ; Leptin - pharmacology ; Leptin - physiology ; Male ; messenger RNA ; Neuropeptides ; Northern blot ; obesity ; orexin ; Orexin Receptors ; Orexins ; prepro-orexin ; Protein Precursors - genetics ; Protein Precursors - physiology ; Rats ; Rats, Sprague-Dawley ; Receptors, G-Protein-Coupled ; Receptors, Neuropeptide - genetics ; Receptors, Neuropeptide - physiology ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; RT-PCR</subject><ispartof>Biochemical and biophysical research communications, 2000-03, Vol.269 (1), p.41-45</ispartof><rights>2000 Academic Press</rights><rights>Copyright 2000 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c461t-9c981a2b1b74a84a1db995a4e1f3210076f8d62c5bbfbf9bd9faa2ae23dcdfab3</citedby><cites>FETCH-LOGICAL-c461t-9c981a2b1b74a84a1db995a4e1f3210076f8d62c5bbfbf9bd9faa2ae23dcdfab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10694474$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>López, Miguel</creatorcontrib><creatorcontrib>Seoane, Luisa</creatorcontrib><creatorcontrib>del Carmen García, María</creatorcontrib><creatorcontrib>Lago, Francisca</creatorcontrib><creatorcontrib>Casanueva, Felipe F.</creatorcontrib><creatorcontrib>Señarís, Rosa</creatorcontrib><creatorcontrib>Diéguez, Carlos</creatorcontrib><title>Leptin Regulation of Prepro-orexin and Orexin Receptor mRNA Levels in the Hypothalamus</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The aim of this study was to determine the effects of leptin treatment on prepro-orexin and orexin receptor expression in the rat hypothalamus. Adult male rats, food-deprived for 48 and 72 h, were treated one time with vehicle or leptin (10 μg, icv). Prepro-orexin mRNA content was measured by semiquantitative RT-PCR, Northern blot, and in situ hybridization; orexin receptor 1 and 2 mRNA content was quantified by Northern blot and/or semiquantitative RT-PCR. Our results indicate that leptin inhibits a fasting-induced increase in prepro-orexin mRNA and orexin receptor 1 mRNA levels in the rat hypothalamus, while orexin receptor 2 mRNA levels were unchanged in all situations evaluated. These data provide direct evidence for an additional mechanism of adaptation of the hypothalamus to food deprivation and for a new effect of leptin in the regulation of food intake.</description><subject>Animals</subject><subject>Eating - drug effects</subject><subject>Eating - genetics</subject><subject>Eating - physiology</subject><subject>fasting</subject><subject>Fasting - physiology</subject><subject>feeding</subject><subject>food deprivation</subject><subject>Food Deprivation - physiology</subject><subject>food intake</subject><subject>Gene Expression Regulation - drug effects</subject><subject>hypothalamus</subject><subject>Hypothalamus - drug effects</subject><subject>Hypothalamus - metabolism</subject><subject>Hypothalamus - physiology</subject><subject>In Situ Hybridization</subject><subject>Intracellular Signaling Peptides and Proteins</subject><subject>leptin</subject><subject>Leptin - pharmacology</subject><subject>Leptin - physiology</subject><subject>Male</subject><subject>messenger RNA</subject><subject>Neuropeptides</subject><subject>Northern blot</subject><subject>obesity</subject><subject>orexin</subject><subject>Orexin Receptors</subject><subject>Orexins</subject><subject>prepro-orexin</subject><subject>Protein Precursors - genetics</subject><subject>Protein Precursors - physiology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, G-Protein-Coupled</subject><subject>Receptors, Neuropeptide - genetics</subject><subject>Receptors, Neuropeptide - physiology</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>RT-PCR</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNqFkE1P3DAQhi3UqizQK8c2p96y9TheZ31EqC1IK6i2UPVm-WMMrpJ4aycI_j1ewqGXqidb7zyeGT-EnAJdAqXiszHJLhmldMkYXx2QBVBJawaUvyGLEouaSfh1SI5y_k0pABfyHTkEKiTnLV-QnxvcjWGotng3dXoMcaiir74n3KVYx4SPpaYHV13P1y3awsdU9durs2qDD9jlquTjPVYXT7s43utO91M-IW-97jK-fz2Pye3XLzfnF_Xm-tvl-dmmtlzAWEsr16CZAdNyveYanJFypTmCb8ofaCv82glmV8Z446Vx0mvNNLLGWee1aY7Jp7lvWffPhHlUfcgWu04PGKesWiob0Uj2XxBaCa1omwIuZ9CmmHNCr3Yp9Do9KaBqr1ztlau9crVXXh58eO08mR7dX_jsuAAfZ8DrqPRdClnd_mAUGsokZ_BCrGei2MSHgEllG3Cw6EJCOyoXw7-mPwOdpZny</recordid><startdate>20000305</startdate><enddate>20000305</enddate><creator>López, Miguel</creator><creator>Seoane, Luisa</creator><creator>del Carmen García, María</creator><creator>Lago, Francisca</creator><creator>Casanueva, Felipe F.</creator><creator>Señarís, Rosa</creator><creator>Diéguez, Carlos</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20000305</creationdate><title>Leptin Regulation of Prepro-orexin and Orexin Receptor mRNA Levels in the Hypothalamus</title><author>López, Miguel ; Seoane, Luisa ; del Carmen García, María ; Lago, Francisca ; Casanueva, Felipe F. ; Señarís, Rosa ; Diéguez, Carlos</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c461t-9c981a2b1b74a84a1db995a4e1f3210076f8d62c5bbfbf9bd9faa2ae23dcdfab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Eating - drug effects</topic><topic>Eating - genetics</topic><topic>Eating - physiology</topic><topic>fasting</topic><topic>Fasting - physiology</topic><topic>feeding</topic><topic>food deprivation</topic><topic>Food Deprivation - physiology</topic><topic>food intake</topic><topic>Gene Expression Regulation - drug effects</topic><topic>hypothalamus</topic><topic>Hypothalamus - drug effects</topic><topic>Hypothalamus - metabolism</topic><topic>Hypothalamus - physiology</topic><topic>In Situ Hybridization</topic><topic>Intracellular Signaling Peptides and Proteins</topic><topic>leptin</topic><topic>Leptin - pharmacology</topic><topic>Leptin - physiology</topic><topic>Male</topic><topic>messenger RNA</topic><topic>Neuropeptides</topic><topic>Northern blot</topic><topic>obesity</topic><topic>orexin</topic><topic>Orexin Receptors</topic><topic>Orexins</topic><topic>prepro-orexin</topic><topic>Protein Precursors - genetics</topic><topic>Protein Precursors - physiology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors, G-Protein-Coupled</topic><topic>Receptors, Neuropeptide - genetics</topic><topic>Receptors, Neuropeptide - physiology</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>RT-PCR</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>López, Miguel</creatorcontrib><creatorcontrib>Seoane, Luisa</creatorcontrib><creatorcontrib>del Carmen García, María</creatorcontrib><creatorcontrib>Lago, Francisca</creatorcontrib><creatorcontrib>Casanueva, Felipe F.</creatorcontrib><creatorcontrib>Señarís, Rosa</creatorcontrib><creatorcontrib>Diéguez, Carlos</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>López, Miguel</au><au>Seoane, Luisa</au><au>del Carmen García, María</au><au>Lago, Francisca</au><au>Casanueva, Felipe F.</au><au>Señarís, Rosa</au><au>Diéguez, Carlos</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Leptin Regulation of Prepro-orexin and Orexin Receptor mRNA Levels in the Hypothalamus</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2000-03-05</date><risdate>2000</risdate><volume>269</volume><issue>1</issue><spage>41</spage><epage>45</epage><pages>41-45</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The aim of this study was to determine the effects of leptin treatment on prepro-orexin and orexin receptor expression in the rat hypothalamus. Adult male rats, food-deprived for 48 and 72 h, were treated one time with vehicle or leptin (10 μg, icv). Prepro-orexin mRNA content was measured by semiquantitative RT-PCR, Northern blot, and in situ hybridization; orexin receptor 1 and 2 mRNA content was quantified by Northern blot and/or semiquantitative RT-PCR. Our results indicate that leptin inhibits a fasting-induced increase in prepro-orexin mRNA and orexin receptor 1 mRNA levels in the rat hypothalamus, while orexin receptor 2 mRNA levels were unchanged in all situations evaluated. These data provide direct evidence for an additional mechanism of adaptation of the hypothalamus to food deprivation and for a new effect of leptin in the regulation of food intake.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>10694474</pmid><doi>10.1006/bbrc.2000.2245</doi><tpages>5</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2000-03, Vol.269 (1), p.41-45 |
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| language | eng |
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| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | Animals Eating - drug effects Eating - genetics Eating - physiology fasting Fasting - physiology feeding food deprivation Food Deprivation - physiology food intake Gene Expression Regulation - drug effects hypothalamus Hypothalamus - drug effects Hypothalamus - metabolism Hypothalamus - physiology In Situ Hybridization Intracellular Signaling Peptides and Proteins leptin Leptin - pharmacology Leptin - physiology Male messenger RNA Neuropeptides Northern blot obesity orexin Orexin Receptors Orexins prepro-orexin Protein Precursors - genetics Protein Precursors - physiology Rats Rats, Sprague-Dawley Receptors, G-Protein-Coupled Receptors, Neuropeptide - genetics Receptors, Neuropeptide - physiology RNA, Messenger - genetics RNA, Messenger - metabolism RT-PCR |
| title | Leptin Regulation of Prepro-orexin and Orexin Receptor mRNA Levels in the Hypothalamus |
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