In vivo PC3 overexpression by retroviral vector affects cell differentiation of rat cortical precursors

The PC3 gene is a marker of dividing neuroepithelial (NE) cells. We transduced single cortical precursors of the ventricular zone (VZ) with a PC3-carrying retroviral vector at E16 stage, and analysed the effects of transgene expression on their progeny in 3-week-old animals. Unlike control-transduce...

Full description

Saved in:
Bibliographic Details
Published in:Brain research. Developmental brain research 2001-06, Vol.128 (2), p.181-185
Main Authors: Malatesta, Paolo, Sgadò, Paola, Caneparo, Luca, Barsacchi, Giuseppina, Cremisi, Federico
Format: Article
Language:English
Subjects:
Animals
Aspartic Acid Endopeptidases - genetics
Asymmetric cell division
Cell Differentiation - physiology
Cell Survival - physiology
Cerebral Cortex - cytology
Cerebral Cortex - embryology
Female
Gene Expression Regulation, Developmental
Gene Expression Regulation, Viral
Genetic Vectors
In vivo
Neural stem cell
Neurogenesis
Neurons - cytology
Neurons - physiology
PC3
PC3 gene
Pregnancy
Proprotein Convertases
Rats
Rats, Sprague-Dawley
Retroviral vector
Retroviridae - genetics
Stem Cells - cytology
Stem Cells - physiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The PC3 gene is a marker of dividing neuroepithelial (NE) cells. We transduced single cortical precursors of the ventricular zone (VZ) with a PC3-carrying retroviral vector at E16 stage, and analysed the effects of transgene expression on their progeny in 3-week-old animals. Unlike control-transduced cells, all viable PC3-transduced cells remained close to the ventricle and displayed a round-shaped, undifferentiated morphology.
ISSN:0165-3806
DOI:10.1016/S0165-3806(01)00170-5