Viability and infectivity of oocysts recovered from clams, Ruditapes philippinarum, experimentally contaminated with Cryptosporidium parvum

This study confirms the important role of marine bivalve molluscs, destined for human consumption, as transmitters of cryptosporidiosis, zoonotic diarrhoeal disease caused by Cryptosporidium parvum. C. parvum oocysts recovered from seawater clams (Ruditapes philippinarum) were viable and infective i...

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Bibliographic Details
Published in:Parasitology research (1987) 2001-06, Vol.87 (6), p.428-430
Main Authors: FREIRE-SANTOS, F, OTEIZA-LOPEZ, A. M, CASTRO-HERMIDA, J. A, GARCIA-MARTIN, O, ARES-MAZAS, M. E
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Biological and medical sciences
Bivalvia - parasitology
Cryptosporidiosis - etiology
Cryptosporidiosis - transmission
Cryptosporidium parvum - isolation & purification
Cryptosporidium parvum - pathogenicity
Fundamental and applied biological sciences. Psychology
Gills - parasitology
Humans
Intestines - parasitology
Life cycle. Host-agent relationship. Pathogenesis
Mice
Parasite Egg Count
Protozoa
Rosaniline Dyes
Staining and Labeling
Water - parasitology
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Summary:This study confirms the important role of marine bivalve molluscs, destined for human consumption, as transmitters of cryptosporidiosis, zoonotic diarrhoeal disease caused by Cryptosporidium parvum. C. parvum oocysts recovered from seawater clams (Ruditapes philippinarum) were viable and infective in five of eight infected neonatal CD-1 Swiss mice. Oocysts were observed in clam gill and gastrointestinal tract tissue homogenates as well as in gill histological sections, by an immunofluorescent antibody technique. In vitro viability of recovered oocysts was also determined using fluorogenic vital dyes (75% viability).
ISSN:0932-0113
1432-1955
DOI:10.1007/s004360100382