Extension of AOAC Official Method 996.01 to the analysis of standard reference Material (SRM) 1846 and infant formulas

• Published in: Journal of AOAC International 2001-05, Vol.84 (3), p.805-813
• Main Authors: SATCHITHANANDAM, Subramaniam, FRITSCHE, Jan, RADER, Jeanne I
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Chromatography, Gas; Fatty Acids - analysis; Food Analysis - methods; Food Analysis - standards; Food industries; Fundamental and applied biological sciences. Psychology; General aspects; Glycine max; Humans; Infant; Infant Food - analysis; Linoleic Acids - analysis; Linolenic Acids - analysis; Methods of analysis, processing and quality control, regulation, standards; Milk; Reference Standards; Reproducibility of Results; Research and development. New food products, dietetic foods and beverages; Triglycerides - analysis
• ISSN: 1060-3271; 1944-7922
• DOI: 10.1093/jaoac/84.3.805

There is currently no official method for the analysis of fatty acids (including trans fatty acids) in infant formulas. AOAC Official Method 996.01 for Fat Analysis in Cereal Products was extended to the analysis of milk-based infant formula Standard Reference Material (SRM)1846 to determine its applicability for use with infant formulas. Following the analysis of SRM 1846, 2 infant formulas, one milk-based liquid and one soy-based powdered infant formula, were analyzed for total fatty acid composition. Fatty acid methyl esters were prepared and analyzed by gas chromatography. The results of the analysis of SRM 1846 show that the mean analyzed values were highly reproducible as indicated by low coefficients of variation (CV). The CVs were